食品科学2024，Vol.45Issue(13)：49-57,9.DOI:10.7506/spkx1002-6630-20230703-017
气相色谱-氢火焰离子化测定脂肪酸甲酯的响应机理与定量规律
Determination of Fatty Acid Methyl Esters by Gas Chromatography-Flame Ionization Detection:Response Mechanism and Quantitative Pattern
摘要
Abstract
In light of the shortcomings of the quantitative methods for the determination of fatty acid methyl esters(FAME)using gas chromatography-flame ionization detection(GC-FID)specified by the current Chinese and international standards,this work systematically studied the response pattern,theoretical model,and experimental interfering factors in the GC-FID method for the determination of 37 FAME through theoretical derivation and laboratory validation from the perspectives of instruments,chromatographic columns,split ratio,and standard substances.Then,the concepts of"quality coefficient"and"correlation factor"were proposed to reveal the functional relationship and equivalence conditions between experimental relevance factors(FFE)and theoretical relevance factors(FFT).Through multiple experiments,the stability of the correlation factors was confirmed.It was found that the theoretical quality coefficient(QCT)and the FFT could be fully utilized for relative or absolute quantification of FAME with more than 10 carbon atoms.The experimental quality coefficient(QCE)and FFE for FAME with less than 10 carbon atoms could be accurately measured through methods such as freshly preparing FAME reference materials and be used to correct the amount of FAME for quantitative analysis.This not only improved the accuracy of quantitative results,but also increased the timeliness of the use of reference materials and reduced the amount of reference materials used.关键词
脂肪酸甲酯/甲酯化/气相色谱-氢火焰离子化检测/质量系数/关联因子Key words
fatty acid methyl esters/methyl esterification/gas chromatography-flame ionization detection/quality coefficient/correlation factor分类
轻工业引用本文复制引用
崔华玲,殷秋妙,杨慧,孙丽华,叶倩,丁晨红,王威利,季天荣,马蕾,曾坤宏..气相色谱-氢火焰离子化测定脂肪酸甲酯的响应机理与定量规律[J].食品科学,2024,45(13):49-57,9.基金项目
广东省企业科技特派员项目(GDKTP2020064600) （GDKTP2020064600）
广东省农产品质量安全风险评估重点实验室项目(2019B121203009) （2019B121203009）
