食品科学2024，Vol.45Issue(13)：115-124,10.DOI:10.7506/spkx1002-6630-20230919-168
红鳍东方鲀鱼皮胶原蛋白制备抗酪氨酸酶活性肽及其活性表征
Preparation and Activity Evaluation of Anti-tyrosinase Peptides from Takifugu rubripes Skin Collagen
摘要
Abstract
Response surface methodology(RSM)was employed to optimize the hydrolysis conditions of acid-soluble collagen(ASC)from Takifugu rubripes skin for the preparation of anti-tyrosinase peptides.The anti-tyrosinase and antioxidant activity of ultrafiltration peptides with different molecular masses were tested before and after being subjected to simulated gastrointestinal digestion,and the moisture absorption and retention properties were also evaluated to explore their application potential.The results showed that neutral protease was the most suitable enzyme for releasing anti-tyrosinase peptides from T rubripes skin collagen.The optimum process conditions were as follows:substrate concentration 2.9 g/100 mL,hydrolysis time 7.8 h,and enzyme dosage 7 862 U/g.The hydrolysate obtained under these conditions inhibited tyrosinase activity by(35.14±0.03)％.The molecular mass of the hydrolysate mainly ranged from 0 to 5 kDa,and the fraction CP-1(＜3 kDa),with the highest anti-tyrosinase activity,scavenged 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation and hydroxyl radicals with half maximal inhibitory concentration(IC50)values of 9.51,2.03 and 7.29 mg/mL,respectively.CP-1 maintained high anti-tyrosinase and antioxidant activity after in vitro simulated digestion.In addition,CP-1 had high moisture absorption and retention properties and therefore could be used as a substitute for glycerol.This study may provide a theoretical basis for the deep processing and application of collagen peptides from T.rubripes skin.关键词
红鳍东方鲀/鱼皮/胶原蛋白/抗酪氨酸酶活性肽Key words
Takifugu rubripes/fish skin/collagen/anti-tyrosinase peptide分类
轻工纺织引用本文复制引用
李晓雅,郭明珠,刘书源,桑亚新,张海恩,孙纪录..红鳍东方鲀鱼皮胶原蛋白制备抗酪氨酸酶活性肽及其活性表征[J].食品科学,2024,45(13):115-124,10.基金项目
河北省重点研发计划项目(22327310D) （22327310D）
河北省现代农业产业技术体系淡水养殖创新团队建设项目(HBCT2023230207) （HBCT2023230207）
