食品与发酵工业2024，Vol.50Issue(13)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.035957
枯草芽孢杆菌无细胞上清液抑制单增李斯特菌生物被膜形成的研究
Inhibitory effect of Bacillus subtilis cell-free supernatant on biofilm formation of Listeria monocytogenes
摘要
Abstract
Listeria monocytogenes is a common foodborne pathogen,and its biofilm is an important cause of food contamination and disease transmission.In this study,the cell free supernatant(CFS)of Bacillus subtilis was selected as an inhibitor to explore the role and potential of B.subtilis CFS in inhibiting L.monocytogenes 118(No.118 for short)biofilm.Firstly,the minimum inhibitory concentration(MIC)of B.subtilis CFS was determined,and then the biofilm inhibition rate of B.subtilis CFS to No.118 was determined by using crys-tal violet staining method.Results showed that CFS at different sub-MICs had a significant inhibitory effect on No.118 biofilm,and the biofilm inhibition rate could still reach 47％at 1/64 MIC.Meanwhile,B.subtilis CFS also significantly inhibited the metabolic activity,self-aggregation rate,surface hydrophobicity,swaming and extramembrane polymer secretion of No.118 biofilm cells.Finally,the changes of No.118 biofilm structure and cell distribution under the action of CFS were observed by confocal laser scanning microscope.After CFS treatment,the structure of No.118 biofilm was loose,and the number of bacteria was significantly reduced.Consequently,this study found that B.subtilis CFS can inhibit the biofilm formation of No.118 biofilm cells by affecting their metabolic activity,self-aggregation ability and swaming,as well as the secretion of extramembrane polymers.This has important reference value for exploring the application of probi-otic metabolites as biofilm inhibitors.关键词
枯草芽孢杆菌/无细胞上清液/单增李斯特菌/生物被膜/激光共聚焦显微镜Key words
Bacillus subtilis/cell free supernatants/Listeria monocytogenes/biofilm/confocal laser scanning microscope引用本文复制引用
胡安祺,杨慧轩,姚现琦,董鹏程,朱立贤,罗欣,张一敏,刘昀阁..枯草芽孢杆菌无细胞上清液抑制单增李斯特菌生物被膜形成的研究[J].食品与发酵工业,2024,50(13):1-8,8.基金项目
国家重点研发政府间国际科技创新合作专项(2019YFE0103800) （2019YFE0103800）
山东省自然科学基金项目(ZR2022QC182) （ZR2022QC182）
现代农业产业技术体系建设专项资金项目(CARS-37) （CARS-37）
