食品与发酵工业2024，Vol.50Issue(13)：9-15,7.DOI:10.13995/j.cnki.11-1802/ts.039058
热带假丝酵母β-紫罗兰酮工程菌的构建及其发酵优化
Construction and fermentation optimization of β-ionone engineered strain in Candida tropicalis
摘要
Abstract
β-ionone is a monoterpene compound found in plants that exhibits various biological activities,including deworming,anti-bacterial,anticancer,and antioxidant properties.It is commonly utilized in agriculture,food,medicine,and flavor industries.Candida tropi-calis is a synthetic chassis cell with the potential for efficient terpenoid production.To enhance the yield of β-ionone,based on the β-ionone engineered strain previously constructed by our research group,this study co-expressed PhCCD1,the key enzyme of β-ionone synthesis,in the cytoplasm,peroxisomes,and lipid droplets through subcellular compartment combination of enzymes.This resulted in the generation of β-ionone engineered strain PCL-01.Subsequently,this study optimized the conditions for shaking flask fermentation,including the liquid amount in the medium,the carbon-to-nitrogen ratio,the fermentation temperature,and the concentration of cofactor Fe2+.Finally,this study conducted a small-scale test in the 5 L bioreactor.The shake flask yield of β-ionone for the engineered strain PCL-01 was 152.4 mg/L,representing a 50％increase compared to the starting strain.After optimizing shake flask fermentation,the yield increased by 22％to 185.8 mg/L.Ultimately,in a 5 L bioreactor,the yield reached 403.9 mg/L.This study is significant for the metabolic transformation of other strains in terpenoid synthesis.It also provides valuable references for researching the fermentation performance of other bacterial species.关键词
热带假丝酵母/β-紫罗兰酮/亚细胞器工程/过氧化物酶体/脂滴/发酵优化/补料分批发酵Key words
Candida tropicalis/β-ionone/subcellular organelle engineering/peroxisomes/lipid droplets/fermentation optimization/fed-batch fermentation引用本文复制引用
徐洁,夏媛媛,沈微,杨海泉,陈献忠..热带假丝酵母β-紫罗兰酮工程菌的构建及其发酵优化[J].食品与发酵工业,2024,50(13):9-15,7.基金项目
国家自然科学基金项目(32271533) （32271533）
