热带假丝酵母β-紫罗兰酮工程菌的构建及其发酵优化OA北大核心CSTPCD

β-ionone is a monoterpene compound found in plants that exhibits various biological activities,including deworming,anti-bacterial,anticancer,and antioxidant properties.It is commonly utilized in agriculture,food,medicine,and flavor industries.Candida tropi-calis is a synthetic chassis cell with the potential for efficient terpenoid production.To enhance the yield of β-ionone,based on the β-ionone engineered strain previously constructed by our research group,this study co-expressed PhCCD1,the key enzyme of β-ionone synthesis,in the cytoplasm,peroxisomes,and lipid droplets through subcellular compartment combination of enzymes.This resulted in the generation of β-ionone engineered strain PCL-01.Subsequently,this study optimized the conditions for shaking flask fermentation,including the liquid amount in the medium,the carbon-to-nitrogen ratio,the fermentation temperature,and the concentration of cofactor Fe2+.Finally,this study conducted a small-scale test in the 5 L bioreactor.The shake flask yield of β-ionone for the engineered strain PCL-01 was 152.4 mg/L,representing a 50％increase compared to the starting strain.After optimizing shake flask fermentation,the yield increased by 22％to 185.8 mg/L.Ultimately,in a 5 L bioreactor,the yield reached 403.9 mg/L.This study is significant for the metabolic transformation of other strains in terpenoid synthesis.It also provides valuable references for researching the fermentation performance of other bacterial species.