食品与发酵工业2024，Vol.50Issue(13)：16-24,9.DOI:10.13995/j.cnki.11-1802/ts.039062
大肠杆菌L-丝氨酸脱水酶的表达改善甘氨酸营养缺陷型毕赤酵母L-丝氨酸的生长
Expression of Escherichia coli L-serine dehydratase improves growth of glycine auxotroph Pichia pastoris on serine
摘要
Abstract
Amino acids cannot be rapidly utilized by microorganisms as a gradually metabolized carbon source.L-serine dehydratase(L-SerDH)catalyzes the one-step conversion of L-serine into pyruvate and ammonia,facilitating central carbon metabolism to generate bi-omass without the consumption of ATP and reducing power.L-serine is a key intermediate in the formate utilization pathway(reductive gly-cine pathway).Therefore,improving the utilization of L-serine can provide valuable insights into the utilization of formate and serine as carbon sources.This paper explored serine tolerance of Pichia pastoris.Results demonstrated that P.pastoris exhibited tolerance to 20 g/L of L-serine.Expression optimization of endogenous L-serine dehydratase was carried out in P.pastoris.Eight heterologous L-serine de-hydratases were expressed separately in glycine auxotroph P.pastoris.The results indicated that L-SerDH encoded by the E.coli tdcG gene had the best effect on the improvement of serine utilization,with the final OD600 increased to 1.6-fold that of the original strain.This study represents the validation of L-serine tolerance in P.pastoris and screens a superior source of L-serine dehydratase.These findings offer valu-able insights into enhancing enzyme sources for serine utilization in P.pastoris.关键词
L-丝氨酸脱水酶/巴斯德毕赤酵母/丝氨酸耐受性/甘氨酸营养缺陷型菌株/L-丝氨酸Key words
L-serine dehydratase/Pichia pastoris/serine tolerance/glycine auxotroph strains/L-serine引用本文复制引用
刘璐瑶,李康,王世杰,白仲虎,杨艳坤,金坚..大肠杆菌L-丝氨酸脱水酶的表达改善甘氨酸营养缺陷型毕赤酵母L-丝氨酸的生长[J].食品与发酵工业,2024,50(13):16-24,9.基金项目
国家自然科学基金项目(32370054) （32370054）
