海红米糠多糖的提取工艺、结构表征及抑制HepG2细胞增殖研究OA北大核心CSTPCD
Study on extraction technology,structure characterization,and inhibition of HepG2 cell proliferation of sea red rice bran polysaccharide
该文主要考察了海红米糠多糖(sea red rice bran polysaccharide,SRBP)的提取工艺、结构表征及其抑制HepG2细胞增殖的情况.以海红米皮糠为原料,考察了不同提取方法对SRBP得率的影响.在单因素的基础上采用响应面法优化了 SRBP的最佳提取工艺.采用苯酚硫酸法和间羟基联苯比色法测定其化学组成,液相色谱法测定分子质量及单糖组成,并用紫外、红外光谱、扫描电镜对其进行结构表征.采用噻唑蓝(methyl thiazolye tetrazolium,MTT)法测定SRBP对HepG2细胞生长的影响.实验结果表明,SRBP的最佳提取工艺条件为酶解时间61 min,料液比(g∶mL)1∶33,酶解温度59 ℃.经测定,SRBP是一种纯度较高的甘露糖吡喃型杂多糖,不含蛋白及核酸物质,表面粗糙、呈片状结构.MTT实验表明,SRBP在800 pig/mL下对HepG2细胞作用24 h的存活率仅为(9.77±0.81)％,表明其具有抑制HepG2增殖的能力.综上可知,提取得到的SRBP具有一定的抗肿瘤活性,为其在饮食干预肝癌等方面提供了理论依据和数据参考.
The objective of this study was to study the extraction technology,structural characterization of sea red rice bran polysac-charide(SRBP)and its inhibition of HepG2 cell proliferation.Taking sea red rice bran as raw material,the effects of different extraction methods on the yield of SRBP were investigated.The optimal extraction process of SRBP was optimized by the response surface method on the basis of a single factor.The chemical composition was determined by phenol-sulfuric acid method and m-hydroxy-biphenyl colorimetry.The molecular weight and monosaccharide composition were determined by liquid chromatography,and the structure was characterized by ultraviolet-visible,fourier transform infrared spectroscopy,and scanning electron microscopy.The effect of SRBP on the growth of HepG2 cells was determined by methyl thiazolye tetrazolium(MTT)assay.The experimental results showed that the optimal extraction conditions of SRBP were an enzymolysis time of 61 min,solid-liquid ratio of 1∶33(g∶mL),and enzymolysis temperature of 59℃.Results showed that SRBP was a high-purity mannose-pyrane-type heteropolysaccharide,which did not contain protein and nucleic acid substances,and had a rough surface and sheet structure.MTT experiment showed that the survival rate of HepG2 cells treated with SRBP at 800 μg/mL for 24 h was only(9.77±0.81)％,indicating that SRBP had the ability to inhibit the proliferation of HepG2 cells.In conclusion,the ex-tracted SRBP has certain anti-tumor activity,which could provide a theoretical basis and data reference for dietary intervention in liver cancer.
陈岑;陈建平;黄文浩;陈博文;钟赛意;李瑞;刘晓菲;宋兵兵;汪卓
广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/广东省海洋食品工程技术研究中心/水产品深加工广东普通高等学校重点实验室/广东省亚热带果蔬加工科技创新中心,广东湛江,524088广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/广东省海洋食品工程技术研究中心/水产品深加工广东普通高等学校重点实验室/广东省亚热带果蔬加工科技创新中心,广东湛江,524088||海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学),辽宁大连,116034
海红米糠多糖提取工艺结构表征HepG2细胞
sea red rice branpolysaccharideextraction technologystructural characterizationHepG2 cells
《食品与发酵工业》 2024 (013)
47-54 / 8
广东省自然科学基金面上项目(2021A1515012455);湛江市科技计划项目(2022A01045,2020A01034);广东海洋大学"南海学者计划"项目(002029002009);广东海洋大学创新强校项目(230419100);广东省普通高校创新团队项目(2021KCXTD021);广东海洋大学优秀学位论文培育项目(040505042203,040505042204)
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