食品与发酵工业2024，Vol.50Issue(14)：1-9,9.DOI:10.13995/j.cnki.11-1802/ts.039106
表达元件优化促进重组胶原蛋白在谷氨酸棒杆菌中的表达
Efficient production of recombinant collagen in Corynebacterium glutamicum by expression elements optimization
摘要
Abstract
Recombinant collagen is a biopolymer with broad potential applications in various fields,such as biomedicine and tissue engineering.The triple-helix structure of collagen imparts unique biological functions and biocompatibilit but also increases the complexity of its expression in microbial systems.In this study,bacterial collagen V-B was used as a model protein to promote the expression of re-combinant collagen in Corynebacterium glutamicum through optimization of expression elements.Firstly,the most efficient promoter tac-R0 was obtained through promoter screening and fermentation duration optimization.Subsequently,the RBS calculator was used to design a mutation library for ribosome binding sites(RBS)and aligned spacing(AS).The RBS and AS with the highest strength were identified,which increased the yield of V-B to 514 mg/L.Furthermore,through the tandem combination of multi-gene expression cassettes,the final yield of V-B increased by 8.4-fold compared to the initial level,reaching 697 mg/L.This study lays a foundation for the industrial produc-tion of recombinant collagen.关键词
重组胶原蛋白/谷氨酸棒杆菌/启动子/核糖体结合位点/串联表达Key words
recombinant collagen/Corynebacterium glutamicum/promoter/ribosome binding sites/multi-gene expression cassettes引用本文复制引用
程逸凡,张萌,许菲..表达元件优化促进重组胶原蛋白在谷氨酸棒杆菌中的表达[J].食品与发酵工业,2024,50(14):1-9,9.基金项目
国家自然科学基金(22078129) （22078129）
