食品与发酵工业2024，Vol.50Issue(14)：50-59,10.DOI:10.13995/j.cnki.11-1802/ts.036576
不同水解方式下陈皮键合态挥发性化合物的比较
Comparison of bound volatile compounds in Citri reticulatae Pericarpium under different hydrolysis methods
摘要
Abstract
To compare the effects of different hydrolysis conditions on the release of bound volatile compounds from Citri reticulatae Pericarpium,the precursor of bound volatile compounds in Citri reticulatae Pericarpium was obtained through adsorption and elution sepa-ration in Amberlite XAD-2 resin,and then hydrolyzed and released under four conditions,including β-glucosidase,pectinase,mixed en-zyme(β-glucosidase and pectinase),and acid.Gas chromatography-mass spectrometry(GC-MS)and gas chromatography-ion migration spectroscopy(GC-IMS)were used for the analysis of released volatile compounds.GC-MS analysis showed that a total of 35 bound volatile compounds were identified from three enzymatic hydrolysis methods.The compounds hydrolyzed by β-glucosidase and mixed enzyme were mainly esters(93.42％and 90.55％,respectively)with methyl benzoate of the highest content,followed by methyl caprylate and other fatty acid methyl esters,and these esters were undetected in free volatile compounds and pectinase hydrolysis,while a total of 50 bound volatile compounds were identified in acid hydrolysis,mainly aldehydes and alcohols with terpene structure.GC-IMS analysis showed that the compounds produced by enzymatic hydrolysis were mainly aldehydes and alcohols,and the compound composition of pectinase hydroly-sis was similar to blank,while the compounds produced by acid hydrolysis were mainly aldehydes,ketones and acids.In conclusion,β-glucosidase was favorable to the release of Citri reticulatae Pericarpium bound volatile compounds than pectinase,and also of milder hy-drolysis condition than acid,thus hydrolysis by β-glucosidase was a better choice for Citri reticulatae Pericarpium to release the bound vol-atile compounds.关键词
陈皮/挥发性化合物/键合态/酶水解/酸水解/β-葡萄糖苷酶Key words
Citri reticulatae Pericarpium/volatile compounds/bound form/enzymatic hydrolysis/acid hydrolysis/β-glucosidase引用本文复制引用
李笛帆,李玉婷,余祥英,杨金易..不同水解方式下陈皮键合态挥发性化合物的比较[J].食品与发酵工业,2024,50(14):50-59,10.基金项目
国家自然科学基金项目(32202011) （32202011）
广东省基础与应用基础研究基金项目(2020A1515110950) （2020A1515110950）
东莞理工学院高层次人才(创新团队)科研启动项目(KCYCXPT2017007) （创新团队）
广东省创新强校创新团队项目(2021KCXTD035) （2021KCXTD035）
