食品与发酵工业2024，Vol.50Issue(14)：290-296,7.DOI:10.13995/j.cnki.11-1802/ts.037497
基于胶体金免疫层析技术同步快速检测奶茶中的三种毒品成分
Simultaneous detection of three related addictive drugs in milk tea based on colloidal gold immunochromatographic strip
摘要
Abstract
A rapid and simultaneous lateral flow immunochromatography(LFIC)method was established based on colloidal gold(CG)for testing three addictive drugs in milk tea,including methamphetamine(MET),ketamine(KET),and cocaine(COC).After optimizing the key parameters,such as the concentration of coated antigen,the combination position of the three analytes,and the immu-noreaction time,the developed method could be used for the simultaneous identification of three target addictive drays in a milk tea sample with a lower concentration could be tested.The limits of detection of MET,KET,and COC were 1.60,1.59,and 1.39 ng/mL,respec-tively.In milk tea samples,the mean recoveries of the three types of mixed reference analytes ranged from 90％to 109％with a relative standard deviation of less than 5％.Briefly,the multiple CG-LFIC method had the advantages of easy operation,fast analysis,high sensi-tivity,and good specificity.It can be an effective method for simultaneous screening addictive drugs in milk tea and provides promising ap-plications in the bio-detection field.关键词
奶茶/毒品/免疫层析/胶体金/同时检测Key words
milk tea/addictive drugs/lateral flow immunochromatography/colloidal gold/simultaneous testing引用本文复制引用
许鹏,徐淑娟,马骉,厉佳丽,苏威,胥天然,周宇欣,徐涵悦,张明洲..基于胶体金免疫层析技术同步快速检测奶茶中的三种毒品成分[J].食品与发酵工业,2024,50(14):290-296,7.基金项目
浙江省基础公益研究计划农业农村项目(LGN22C20015) （LGN22C20015）
国家级大学生创新创业训练计划资助项目(202310356048) （202310356048）
浙江省大学生科技创新活动计划项目(2023R409A026) （2023R409A026）
