食品与发酵工业2024,Vol.50Issue(14):328-333,6.DOI:10.13995/j.cnki.11-1802/ts.037020
基于金纳米粒子荧光探针可视化快速检测复杂样品中氰化物
Visualized and rapid detection of cyanide in complex samples using fluorescence probes based on gold nanoparticles
摘要
Abstract
This study utilized water-soluble fluorescein isothiocyanate(FITC)to cover graphene loaded gold nanoparticle composites,and the fluorescence of the probe molecule FITC was quenched.Then,by adding cyanide to the system,the strong complexation of cyanide ions(CN-)with gold nanoparticles formed a stable complex Au(CN)3_x,gradually restoring the fluorescence of the probe molecules,thereby achieving rapid detection of cyanide.The pH value of the experimental solution,reaction time,and interfering ions were opti-mized.The experimental results indicated that the FITC-Au NPs fluorescence probe had a minimum detection limit of 0.036 μmol/L for CN-in the presence of an H2O2 environment.There was a good linear relationship within the 0.05-80 μmol/L concentration range.The high stability and selectivity of FITC-Au NPs probes were utilized to achieve rapid detection of CN-in complex samples.This simple,fast,and economical fluorescence sensing system shows great practical potential in the detection of anions in actual samples.关键词
荧光探针/氰化物/杏仁露/异硫氰酸酯/金纳米粒子Key words
fluorescence probe/cyanide/almond dew/isothiocyanate ester/Au nanoparticle引用本文复制引用
马文娟,李蕊岑,袁彩霞,洪霞,王玉,何海宁..基于金纳米粒子荧光探针可视化快速检测复杂样品中氰化物[J].食品与发酵工业,2024,50(14):328-333,6.基金项目
甘肃省市场监督管理局科技计划项目(SSCJG-SP-A202202) (SSCJG-SP-A202202)