食品与机械2024，Vol.40Issue(6)：164-169,6.DOI:10.13652/j.spjx.1003.5788.2023.60197
核桃叶及其乙醇提取物化学成分分析
Chemical composition analysis of walnut leaves and their ethanol extracts
摘要
Abstract
[Objective]This study aimed to investigate the chemical constituents of walnut leaves.[Methods]The volatile components of walnut leaves were analyzed by head-space solid micro-extraction and coupled with GC-MS.The chemical composition of ethanol extract was identified using ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high resolution mass spectrometry(UPLC-Q-Exactive).[Results]88 kinds of volatile compounds were identified in walnut leaves,mainly terpenoids,of which sesquiterpenoids account for 78.35％.30 kinds of active components were identified from ethanol extracts,including twenty kinds of polyphenols(twelve kinds of flavonoids),four kinds of phenylpropanoids,three kinds of pentacyclic triterpenoids,two kinds of essential amino acids and 1 tricarboxylic acid compounds in the ethanol extract in walnut leaves.[Conclusion]The combined use of SPME-GC-MS and UPLC-Q Exactive technology can determine and analyze the chemical composition of walnut leaves systematically,accurately and rapidly.关键词
核桃叶/固相微萃取/气相色谱—质谱法/超高效液相色谱—四极杆—静电场轨道阱高分辨质谱联用技术/化学成分Key words
walnut leaves/solid phase microextraction(SPME)/gas chromatography-mass spectrometry(GC-MS)/ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-exactive)/chemical composition引用本文复制引用
何佳丽,胡恩明,王纪辉,胡伯凯,刘亚娜..核桃叶及其乙醇提取物化学成分分析[J].食品与机械,2024,40(6):164-169,6.基金项目
贵州省科技基础研究计划项目(编号:黔科合基础[2019]1184号) （编号:黔科合基础[2019]1184号）
贵州省科技计划项目(编号:黔科合支撑[2022]重点014号) （编号:黔科合支撑[2022]重点014号）
贵州省科技条件与服务能力建设项目(编号:黔科合服企[2020]4010) （编号:黔科合服企[2020]4010）
