生物技术通报2024，Vol.40Issue(7)：90-98,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0118
基于CRISPR/Cas12a的生物传感平台的机制研究及应用
Mechanism Study and Application of CRISPR/Cas12a-based Biosensing Platform
陈墨岩 1祝诚1
作者信息
- 1. 天津大学生命科学学院,天津 300072
- 折叠
摘要
Abstract
CRISPR/Cas12a system can accurately identify specific single-stranded DNA or double-stranded DNA containing PAM sequences with programmable guide RNA.Then,the non-specific single strand DNA can be cleaved rapidly at the same time as the target substrate,which shows great prospects in biosensing.In recent years,the CRISPR/Cas12a system has been widely used for assisted detection of biomarkers,and its biomarker sensing platform has been applied in the construction of biomolecular sensors such as visualized cell pathways and in vivo diagnostics.Based on the principles of CRISPR/Cas12a biosensing platform construction,this paper summarizes the variants of CRISPR/Cas12a system under different application scenarios,then highlights and summarizes the applications of in vitro and in vivo sensing platforms based on this system,and further discusses the characteristics of different sensing platforms.Finally,the paper summarizes and prospects the advantages and limitations of current applications,aiming to provide a certain reference for the research and application in this field.关键词
CRISPR/Cas12a/体外生物传感/体内生物传感/准确识别Key words
CRISPR/Cas12a/biosensing in vivo/biosensing in vitro/accurately identifying引用本文复制引用
陈墨岩,祝诚..基于CRISPR/Cas12a的生物传感平台的机制研究及应用[J].生物技术通报,2024,40(7):90-98,9.
