利用代谢工程在酿酒酵母中高效合成2-萘乙醇OA北大核心CSTPCD

[Objective]To elucidate the biosynthesis of 2-naphthaleneethanol and explore the gene combination beneficial to the synthesis of 2-naphthaleneethanol.[Method]2-Naphthylalanine was added to the fermentation broth of Saccharomyces cerevisiae BY4741 and the product was detected by HPLC.The growth curve and yield curve of BY4741 were explored.After adding different concentrations of 2-naphthylalanine into the fermentation broth of BY4741,the growth and production level were detected.The plasmids overexpressing six genes of Ehrlich pathway were constructed and transformed into BY4741.Six modified strains were fermented and the product yield was detected.[Result]2-Naphthaleneethanol was detected in the fermentation broth as expected.Compared with the wild type BY4741,the yields of the metabolically engineered strains were all improved.Overexpressing ARO9 and ARO10 made the yield of 2-naphthaleneethanol reached 0.258 mmol/L when exogenous 2-naphthylalanine was added,and the transformation rate reached 2.3 times that of the wild type.[Conclusion]The biosynthesis of 2-naphthaleneethanol was achieved in S.cerevisiae.ARO9 and ARO10 were found to be the optimal gene combinations beneficial to the biosynthesis of 2-naphthaleneethanol.This study provides a new biosynthesis method for industrial production of 2-naphthaleneethanol.