褐角苔FfCYP98基因克隆及其功能分析OA北大核心CSTPCD

[Objective]Cytochrome P450 monooxygenase 98(CYP98)is a key rate-limiting enzyme in the phenylpropanoid pathway,and it is proposed to investigate whether it is involved in the biosynthesis of the phenylpropanoid pathway and in the anti-disease function in Hornworts,so as to provide a reference for the future study of the evolution of the early terrestrial plants and physiological mechanisms of adaptation to adversity stress.[Method]The full-length sequence of FfCYP98 cDNA was cloned from Folioceros fuciformis using RACE,analyzed for bioinformatics and subcellular localization,and functionally verified by constructing an overexpression vector to transform the Arabidopsis thaliana mutant FfCYP98-OE1.[Result]The FfCYP98 cDNA sequence,with an open reading frame of 1305 bp,is evolutionarily closest to the Bryophytes Anthoceros angustus and Physcomitrium patens CYP98,and subcellular localization results show that FfCYP98 is localized to both the nucleus and the cytoplasm.The overexpression of the FfCYP98 gene revealed that the expressions of total phenolics,total flavonoids,and C4H,4CL and C3H in the phenylpropanoid pathway was significantly higher in FfCYP98-OE1 plants than in A.thaliana WT plants.In addition,infestation of F.fuciformis and A.thaliana FfCYP98-OE1 plants with Botrytis cinerea revealed that the expression of FfCYP98 was significantly up-regulated,that FfCYP98-OE1 plants died significantly slower than WT plants,and that the expressions of total phenolics,total flavonoids,and four genes,C4H,HCT,C3H,and CAD in the phenylpropanoid pathway were significantly higher in A.thaliana FfCYP98-OE1 plants than in WT plants.[Conclusion]FfCYP98 may be involved in the biosynthesis of phenolics and flavonoids in the phenylpropanoid pathway by regulating the expressions of genes related to the synthesis of phenylpropanoid pathway,and may be related to disease resistance of plants.