不同雌激素水平大鼠舌下神经核中ERα的表达OACSTPCD
Expression of ERα in hypoglossal nucleus in rats with different estrogen levels
目的 研究雌性大鼠去卵巢对雌激素受体ERα在舌下神经核表达的影响.方法 选取90只8周龄雌性SD大鼠,体质量(250±25)g,随机等量分为假手术组(sham组)、去卵巢组(OVX组)、去卵巢+雌二醇组(OVX+E2组),建立不同雌激素水平大鼠模型.sham组去除大鼠卵巢周围少量脂肪,OVX组去除卵巢,OVX+E2组去除卵巢后皮下注射雌二醇;使用免疫组织化学检测ERα在舌下神经核表达;real-time PCR、Western blot检测ERα mRNA、蛋白表达.结果 三组大鼠舌下神经核均存在ERα阳性表达.与sham组比较,OVX组ERα mRNA及蛋白含量明显降低(P<0.01);与OVX组比较,OVX+E2组ERα mRNA及蛋白含量明显升高(P<0.01);sham组和OVX+E2组间差异无统计学意义(P>0.05).结论 雌激素受体ERα在不同雌激素水平雌性大鼠舌下神经核均存在表达,ERα mRNA及蛋白的表达均受雌激素水平调节.
Objective To study the effect of ovariectomy on the expression of estrogen receptor ERα in the hypoglossal nucleus of female rats.Methods Ninety 8-week-old female SD rats,weighing(250±25)g,were randomly and equally divided into sham ope-ration group(sham group),ovariectomized group(OVX group),and ovariectomized plus estradiol group(OVX+E2 group)to establish different estrogen level rat models.The rats in sham group were performed the removal of a small amount of fat around the ovary,the rats in OVX group were performed the removal of the ovary,and the rats in OVX+E2 group were subcutaneously injected with estradiol after ovariectomy.Immunohistochemistry was used to detect the expression of ERα in the hypoglossal nucleus.Real-time PCR and Western blot were used to detect the expressions of ERα mRNA and protein.Results ERα showed positive expression in the hypo-glossal nucleus of rats in all three groups.Compared with sham group,the mRNA and protein expressions of ERα were significantly reduced in OVX group(P<0.01).Compared with OVX group,the mRNA and protein levels of ERα were significantly increased in OVX+E2 group(P<0.01).There was no statistically significant difference in ERα expression between sham group and OVX+E2 group(P>0.05).Conclusion Estrogen receptor ERα is expressed in the hypoglossal nucleus of female rats with different estrogen levels,and the levels of ERα mRNA and protein are regulated by estrogen level.
李环;梁军;侯玉霞
西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,西安 710004||西安交通大学口腔医院陕西省牙颌疾病临床研究中心
临床医学
去卵巢雌激素舌下神经核ERα大鼠
ovariectomyestrogenhypoglossal nucleusERαrats
《山西医科大学学报》 2024 (006)
735-739 / 5
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