不同雌激素水平大鼠舌下神经核中ERα的表达OACSTPCD

Objective To study the effect of ovariectomy on the expression of estrogen receptor ERα in the hypoglossal nucleus of female rats.Methods Ninety 8-week-old female SD rats,weighing(250±25)g,were randomly and equally divided into sham ope-ration group(sham group),ovariectomized group(OVX group),and ovariectomized plus estradiol group(OVX+E2 group)to establish different estrogen level rat models.The rats in sham group were performed the removal of a small amount of fat around the ovary,the rats in OVX group were performed the removal of the ovary,and the rats in OVX+E2 group were subcutaneously injected with estradiol after ovariectomy.Immunohistochemistry was used to detect the expression of ERα in the hypoglossal nucleus.Real-time PCR and Western blot were used to detect the expressions of ERα mRNA and protein.Results ERα showed positive expression in the hypo-glossal nucleus of rats in all three groups.Compared with sham group,the mRNA and protein expressions of ERα were significantly reduced in OVX group(P＜0.01).Compared with OVX group,the mRNA and protein levels of ERα were significantly increased in OVX+E2 group(P＜0.01).There was no statistically significant difference in ERα expression between sham group and OVX+E2 group(P＞0.05).Conclusion Estrogen receptor ERα is expressed in the hypoglossal nucleus of female rats with different estrogen levels,and the levels of ERα mRNA and protein are regulated by estrogen level.