沈阳农业大学学报2024，Vol.55Issue(4)：417-425,9.DOI:10.3969/j.issn.1000-1700.2024.04.004
贝莱斯芽孢杆菌SF18-3的鉴定及对辣椒细菌性斑点病的防效评价
Identification and Evaluation of Bacillus subtilis SF18-3 Against Bacterial Spot Disease of Pepper
摘要
Abstract
In recent years,bacterial spot disease of pepper[Xanthomonas campestris pv.vesicatoria(Doidge)Dye]has occurred seriously in Liaoning province.In this study,a strain of biocontrol bacterium SF18-3,known for its high efficacy,was obtained from a mixture of animal manure using the dilution plate coating method.The target organism for this isolation was bacterial mottle blotch BWB3 in pepper plants.To further clarify the biocontrol effect of SF18-3,the optimal fermentation conditions were optimized by antibacterial zone method,and the 6 extracellular enzyme activities were determined by double antibody sandwich method.The antibacterial activities were determined in laboratory test and the control efficiency were evaluated in pod experiment.The taxonomic status of SF18-3 was determined by morphological characteristics observation,physiological and biochemical tests and 16S rDNA sequence analysis.The results showed that SF18-3 had the best bacterial inhibition effect after fermentation for 84 h at 30℃with NB as the basal medium at 140 r·min-1.The protease activity and xylanase activity in the fermentation broth were higher,which were 536.13 U·L-1 and 564.22 U·L-1,respectively.Under optimal fermentation conditions,the diameter of the transparent circle was 3.40 cm on the plate and a 70.17% control efficiency on potting.SF18-3 was rod-shaped,spore,Gram-positive bacterium,and 16Sr DNA sequence was clustered with Bacillus velezensis OK169608 and B.velezensis OK147645.According to colony morphology and physiological and biochemical characteristics,strain SF18-3 was identified as B.velezensis.关键词
辣椒细菌性斑点病/贝莱斯芽孢杆菌/胞外酶/防治效果/鉴定Key words
bacterial spot disease of pepper/Bacillus velezensis/extracellular enzymes/control efficiency/identification分类
农业科技引用本文复制引用
王辉,刘丽,于舒怡,关天舒,邹春蕾,李柏宏,郑丽娇..贝莱斯芽孢杆菌SF18-3的鉴定及对辣椒细菌性斑点病的防效评价[J].沈阳农业大学学报,2024,55(4):417-425,9.基金项目
沈阳市中青年科技创新人才支持计划项目(RC210404) （RC210404）
沈阳市农村科技特派团专项项目(23-411-2-19) （23-411-2-19）
