武警医学2024，Vol.35Issue(7)：593-596,601,5.
神经调节蛋白4对2型糖尿病小鼠主动脉内皮细胞凋亡的影响
Effects of neuregulin 4 on apoptosis of aortic endothelial cells in type 2 diabetic mice
摘要
Abstract
Objective To investigate the effects of neuregulin 4(Nrg4)on the apoptosis of endothelial cells in type 2 diabetic mice.Methods C57 mice were randomly divided into Vehicle group,diabetes mellites(DM)group and Nrg4 administration group(NRG4).Nrg4 group was intraperitoneally(i.p.)injected with Nrg4 recombinant protein(100 μg/kg,3 times/week)and the same volume of saline was i.p.injected in the other two groups for 8 weeks.After the experiment,TUNEL staining was used to evaluate ap-optotic rate of endothelial cells,and Western blot was used to investigate the protein levels of apoptosis-associated proteins.Results Compared with Vehicle group,DM significantly promoted cell apoptosis[(33.0±5.4)%vs.(20.5±3.0)%;P<0.05],in-creased Bax and cleaved-caspase 3 expression,and decreased Bcl-2 expression(P<0.05),while Nrg4 administration obviously de-creased endothelial apoptosis[(19.2±3.8)%vs.(33.0±5.4)%;P<0.05],decreased Bax and cleaved-caspase 3 expression,and increased Bcl-2 expression(P<0.05).Conclusions Nrg4 administration can alleviate the apoptosis of aortic endothelial cells in type 2 diabetic mice.关键词
神经调节蛋白4/糖尿病/内皮细胞/细胞凋亡Key words
neuregulin 4/diabetes mellitus/endothelial cell/cell apoptosis分类
医药卫生引用本文复制引用
刘星宇,王莹,焦婷,方文灿,于汉英,张海松..神经调节蛋白4对2型糖尿病小鼠主动脉内皮细胞凋亡的影响[J].武警医学,2024,35(7):593-596,601,5.基金项目
军队某课题资助 （）
