雪腐微座孢引起新疆小麦雪腐病的病原鉴定及生物学特性分析
Pathogen identification and biological characteristics analysis of wheat snow rot caused by Microdochium nivale
摘要
Abstract
[Objective]The study aims to investigate the species and biological characteristics of the causal agents of wheat snow mold disease in the main wheat-producing areas of Yili Kazak Autonomous Pre-fecture(Yili Prefecture),Xinjiang Uygur Autonomous Region,China,in order to provide scientific basis for the development of pesticides and the prevention and control of the disease.[Methods]The pathogen of win-ter wheat snow mold disease was isolated by tissue culture method and inoculated onto healthy wheat plants to verify its pathogenicity.The species of the pathogen was identified by combining its biological characteristics and ITS gene sequence analysis.The biological characteristics of the pathogen were studied by the method of growth rate.[Results]The isolates YLXF-1,YLXF-2,and YLXF-3 could infect healthy wheat and ex-hibit the same symptoms of disease in the field.Morphological and molecular biological identification results indicated that the pathogen was Microdochium nivale.The pathogen could grow at temperatures of 5-35℃,pH values of 3-12,under different light conditions,and on various test media,and could utilize most of the tested carbon and nitrogen sources,indicating strong environmental adaptability.The pathogen had faster my-celial growth at 20℃,pH 8,and 12 h of light-dark alternation culture on PDA medium,and utilized sucrose and beef extract as the better carbon and nitrogen sources,respectively.[Conclusion]The pathogen of wheat snow mold disease in the main production area of winter wheat in Yili,Xinjiang is M.nivale.The optimal growth temperature of the pathogen mycelium is 20℃,pH value is 8,light is 12 h of light and darkness alter-nation,culture medium is PDA,the best carbon source is sucrose,and the best nitrogen source is beef ex-tract.关键词
小麦/雪腐病/病原鉴定/生物学特性Key words
wheat/snow rot/pathogen identification/biological characteristics分类
农业科技引用本文复制引用
王莉,雷斌,周小云,鄢蓉,张军高,李进,梁晶,龚静云,杜雨,马德英..雪腐微座孢引起新疆小麦雪腐病的病原鉴定及生物学特性分析[J].新疆农业科学,2024,61(5):1201-1208,8.基金项目
中央引导地方项目"'一带一路'国际绿色农药研制及应用技术创新基地建设"(ZYYD2023B06) （ZYYD2023B06）
省部共建课题"干旱半干旱地区旱地小麦提质增效关键技术研究"(SBGJXTZXKF-9) （SBGJXTZXKF-9）
新疆维吾尔自治区乡村振兴产业发展科技行动项目"春小麦增密抗逆增产关键技术引进与示范"(2022NC078) （2022NC078）
新疆维吾尔自治区科技特派员农村科技创业行动项目"春小麦高产创建关键技术集成与示范"(2022BKZ022) The central government guides local projects"Construction of'Belt and Road'international green pesticide development and applica-tion technology innovation base"(ZYYD2023B06) （2022BKZ022）
Provincial and ministerial joint projects"Study on Key techniques of improving quality and efficiency of dryland wheat in arid and semi-arid areas"(SBGJXTZXKF-9) （SBGJXTZXKF-9）
Agriculture,Rural Areas and Farmers Backbone Talent Training Project of Xinjiang Uygur Autonomous Region"Introduction and demonstration of key technologies for in-creasing density,stress resistance,and yield of spring wheat"(2022NC078) （2022NC078）
Xinjiang Uygur Autonomous Region Science and Tech-nology Commissioner Rural Science and Technology Entrepreneurship Action Project"Integration and Demonstration of Key Technol-ogies for High Yield Creation of Spring Wheat"(2022BKZ022)" （2022BKZ022）
