新疆农业科学2024，Vol.61Issue(5)：1218-1226,9.DOI:10.6048/j.issn.1001-4330.2024.05.020
基于非靶向代谢组学分析两种酵母培养物的成分差异
Analysis of the composition differences between two yeast cultures based on untargeted metabolomics
摘要
Abstract
[Objective]This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture.[Methods]The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures.[Results](1)Under the super classification annotation,a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids,nucleo-sides,nucleotides and their derivatives,amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures,only significant differences in content(P<0.05).(2)In the positive ion mode,237 differential metabolites were detected,of which 176 were significantly up-regulated(P<0.05)and 61 were significantly down-regulated(P<0.05)in expression.In the negative ion mode,136 differential metabolites were detected,of which 64 were significantly up-regulated(P<0.05)and 72 were significantly down-regulated(P<0.05)in expression.(3)The KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment pathway of differential metabolites mainly focused on alanine,aspartate and glutamate metabolism.[Conclusion]Due to different carbon and nitrogen sources and fermentation conditions,the yeast cultures have significant differences in the content of metabolites,but with their own advantages of the biologi-cal activity of main differential metabolites.关键词
酵母培养物/非靶标代谢组学/差异代谢物/酿酒酵母Key words
yeast culture/untargeted metabolomics/differential metabolite/Saccharomyces cerevisiae分类
农业科技引用本文复制引用
林青,霍向东,时红玲,秦新政,李月,王子涵,高雁,曾军,王浩中,娄恺..基于非靶向代谢组学分析两种酵母培养物的成分差异[J].新疆农业科学,2024,61(5):1218-1226,9.基金项目
新疆维吾尔自治区自然科学基金重点项目(2023D01D11) （2023D01D11）
新疆生产建设兵团重点领域科技攻关计划(2022AB013) （2022AB013）
新疆生产建设兵团重点领域科技攻关计划(2020AB013) Key Program of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2023D01D11) （2020AB013）
Key program of scientific and technological research plan of Xinjiang production and construction corps(2022AB013) （2022AB013）
Key program of scientific and technological research plan of Xinjiang production and construction corps(2020AB013) （2020AB013）
