基于非靶向代谢组学分析两种酵母培养物的成分差异OA北大核心CSTPCD

[Objective]This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture.[Methods]The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures.[Results](1)Under the super classification annotation,a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids,nucleo-sides,nucleotides and their derivatives,amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures,only significant differences in content(P<0.05).(2)In the positive ion mode,237 differential metabolites were detected,of which 176 were significantly up-regulated(P<0.05)and 61 were significantly down-regulated(P<0.05)in expression.In the negative ion mode,136 differential metabolites were detected,of which 64 were significantly up-regulated(P<0.05)and 72 were significantly down-regulated(P<0.05)in expression.(3)The KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment pathway of differential metabolites mainly focused on alanine,aspartate and glutamate metabolism.[Conclusion]Due to different carbon and nitrogen sources and fermentation conditions,the yeast cultures have significant differences in the content of metabolites,but with their own advantages of the biologi-cal activity of main differential metabolites.