封育对荒漠草原真菌群落的影响OA北大核心CSTPCD

[Objective]Assess the response of closure to fungal communities and to describe the role of fungal communities in the soil.[Methods]A field survey was conducted to investigate the effect of sealing on the fungal communities of desert grasslands based on the internal transcribed spacer(ITS)technique and liq-uid chromatography-mass spectrometry(LC-MS),using 14 years of sealing and adjacent free-grazing des-ert grasslands as the study objects.[Results]The results showed that sealing increased the community abun-dance of Ascomycota and decreased the community abundance of Basidiomycota,Mortierellomycota,and un-classified_k_Fungi,significantly altered the beta diversity of the fungal community.In addition,soil nutrient content and plant biomass were higher under the sealing treatment than under the grazing treatment.The fungal communities were more closely linked internally under closed conditions,with the main relationships being competitive with each other,whereas under grazing conditions the fungal communities were mainly in a mutual-ly beneficial symbiotic relationship.A strong correlation between fungal communities and total soil potassium and plant biomass.Soil differential fungal communities were mostly significantly and positively correlated with metabolites.[Conclusion]Sealing altered fungal community richness and increased plant biomass above and below ground.