鸡circMICAL2的鉴定、组织表达谱分析及其功能预测OA北大核心CSTPCD

[Objective]The purpose of this study is to verify the authenticity of chicken circular RNA(circMICAL2)and explore the function and regulation mechanism of circMICAL2 in chicken muscle growth and development.[Methods]Specific primers based on circMICAL2 circular sequences were designed by PCR amplification,and RNase R and actinomycin D treatments were performed to detect the endogenous sta-bility of circMICAL2.Eight tissue samples from heart,liver,lung,kidney,intestine,skin,chest muscle and leg muscles of 16-day-old and 180-day-old chickens(adult chickens)were collected,and the tissue ex-pression profile of circMICAL2 in different development periods was completed by real-time PCR(qRT-PCR);Bioinformatics was used to predict circMICAL2-targeted miRNA and mRNA,and to carry out GO function and KEGG pathway enrichment analysis.[Results]Chicken circMICAL2 really existed,and its cycli-zation stability was strong;circMICAL2 was widely expressed in all tissues of adults and chicks,and circMI-CAL2 was most highly expressed in their chest and leg muscles of adults and chicks,and the expression of cir-cMICAL2 in both adult leg and thorax muscles was significantly higher than that in chicks(P<0.05),indica-ting that circMICAL2 was closely related to the regulation of chicken muscle growth and development.circMI-CAL2 targets gga-miR-103-3p and gga-miR-130b-3p regulated 225 downstream potential target genes.The target genes of circMICAL2 were mainly enriched in TGF-β signaling pathway,MAPK signaling pathway,cell cycle and other related signaling pathways.[Conclusion]Chicken circMICAL2 really exists,and the expression of circMICAL2 plays an important role in regulating the growth of muscle during different growth and development periods.