胰淀素作用于背外侧被盖核胆碱乙酰转移酶神经元调节小鼠体重OA北大核心CSTPCD

Amylin acts on the lateral dorsal tegmental nucleus(LDT),induces weight loss and diet-suppressive effects,and activates dorsal scapular brown adipose tissue(IBAT)to produce heat.Multiple appetite hormone receptors colocalized with choline acetyltransferase(ChAT)neurons in LDT to modulate saturation and energy expenditure.However,it remains to be determined whether ChAT neurons in LDT are involved in amylin-mediated weight regulation.Immunofluorescence was conducted to detect the expression of c-Fos and ChAT neurons in mouse LDT,retrograde tracer analysis was selected to confirme the downstream nucleus mass of LDT,and neuronal damage assays was conducted to analyze the neuromechanism of amylin action.Results showed that intraperitoneal administration of amylin significantly reduced the body weight of mice and significantly increased the expression of c-Fos and ChAT neurons in both the LDT and parabrachial nucleus(PBN).ChAT and neuronal nitric oxide synthase(nNOS)neurons were co-expressed in the dorsal motor nucleus of the vagus(DMV).Retrograde tracer experiments demonstrated that LDTs send nerve projections to PBN neurons.Furthermore,injury of LDT and PBN neurons reduced the weight loss of amylin.LDT is the center of energy balance regulation connected to PBN,and the ChAT neurons of this circuit are involved in the process of weight regulation of amylin.