畜牧兽医学报2024，Vol.55Issue(7)：3185-3192,8.DOI:10.11843/j.issn.0366-6964.2024.07.036
胰淀素作用于背外侧被盖核胆碱乙酰转移酶神经元调节小鼠体重
Amylin Acts on Choline Acetyltransferase Neurons in the Lateral Dorsal Tegmental Nucleus(LDT)to Regulate Body Weight of Mice
摘要
Abstract
Amylin acts on the lateral dorsal tegmental nucleus(LDT),induces weight loss and diet-suppressive effects,and activates dorsal scapular brown adipose tissue(IBAT)to produce heat.Multiple appetite hormone receptors colocalized with choline acetyltransferase(ChAT)neurons in LDT to modulate saturation and energy expenditure.However,it remains to be determined whether ChAT neurons in LDT are involved in amylin-mediated weight regulation.Immunofluorescence was conducted to detect the expression of c-Fos and ChAT neurons in mouse LDT,retrograde tracer analysis was selected to confirme the downstream nucleus mass of LDT,and neuronal damage assays was conducted to analyze the neuromechanism of amylin action.Results showed that intraperitoneal administration of amylin significantly reduced the body weight of mice and significantly increased the expression of c-Fos and ChAT neurons in both the LDT and parabrachial nucleus(PBN).ChAT and neuronal nitric oxide synthase(nNOS)neurons were co-expressed in the dorsal motor nucleus of the vagus(DMV).Retrograde tracer experiments demonstrated that LDTs send nerve projections to PBN neurons.Furthermore,injury of LDT and PBN neurons reduced the weight loss of amylin.LDT is the center of energy balance regulation connected to PBN,and the ChAT neurons of this circuit are involved in the process of weight regulation of amylin.关键词
肥胖/胆碱乙酰转移酶/胰淀素/下丘脑/背外侧被盖核Key words
obesity/choline acetyltransferase/amylin/hypothalamus/lateral dorsal tegmental nucleus分类
畜牧业引用本文复制引用
曹晓娟,陈玉洁,张小宇,海日汗,杜晨光,刘昊东,李鹏辉,李嘉成,樊奇,王星,李彩琴,杨子程,郭永清..胰淀素作用于背外侧被盖核胆碱乙酰转移酶神经元调节小鼠体重[J].畜牧兽医学报,2024,55(7):3185-3192,8.基金项目
国家自然科学基金(32060204) （32060204）
内蒙古科技攻关计划(2021GG0199) （2021GG0199）
内蒙古自然科学基金(2020MS03035) （2020MS03035）
内蒙古自治区教育厅高等学校科学研究项目(NJZY22535) （NJZY22535）
内蒙古自治区直属高校基本科研业务费项目(BR220150) （BR220150）
内蒙古自治区高等学校科学研究重点项目(NJZZ22529) （NJZZ22529）
内蒙古自治区教育厅高等学校科学研究项目(NJZY23120) （NJZY23120）
