畜牧与兽医2024，Vol.56Issue(8)：15-20,6.
中国荷斯坦奶牛CSN3基因多态性检测及其与产奶性状的关联分析
Detection of CSN3 gene polymorphisms and its association with milk production traits in Chinese Holstein cattle
摘要
Abstract
In order to identify the polymorphism of the CSN3 gene and analyze their effects on dairy production traits and milk quality of Chinese Holstein cattle in Anhui Provence,DNA sequencing was performed,in this study,to detect SNPs in 402 Chinese Holstein cattle.Population genetic information analysis and the correlation of genotype and production traits were conducted.Two loci of the CSN3 gene in 402 Chinese Holsteincattle(g.13068C＞T,g.13124A＞G)were found.In the g.13068C＞T locus,the genotype frequency of CC genotype cows at the g.13068C＞T locus was 0.52;the CC genotype cattle had a significantly higher average daily milk production than the CT geno-type dairy cattle(P＜0.01).The TT genotype dairy cattle had significantly higher milk fat percentage than did the CC genotype dairy cattle(P＜0.01).The milk fat content of the CT genotype cattle was higher than that of the CC genotype cattle(P＜0.05).The protein content of milk was highest in the CT genotype cattle;the milk urea nitrogen(MUN)and WHI were higher in the TT genotype cattle,compared with those in the other two genotypes,but with no significant differences.In the g.13124A＞G locus,the dairy cattle with the AA genotype cattle had significantly higher milk protein percentage than did the AG genotype dairy cattle(P＜0.05).The daily average milk production of the AG genotype cattle was higher than that of the AA genotype cattle.The milk fat content of the AA genotype cattle was higher than that of the AG genotype cattle.The somatic cell count of the AG genotype cattle was higher than that of the AA genotype cattle.The MUN and WHI in the milk of the AA genotype cattle were higher than those of the AG genotype cattle,but with no significant differences.In summary,the g.13068C＞T locus might be used as a molecular marker for milk production and fat rate in Chinese Holstein dairy cattle,the g.13124A＞G locus might be used as a molecular marker for protein content of milk in Chinese Holstein dairy cattle,and the CSN3 gene could be a major or candidate gene influencing milk production performance in Chinese Holstein cattle.关键词
中国荷斯坦奶牛/CSN3基因/单核苷酸突变/分子标记Key words
Chinses Holstein cattle/CSN3 gene/SNP/molecular marker分类
农业科技引用本文复制引用
吴涛,齐云霞,朱洪龙,黄冬维,赵辉玲,程广龙,赵小伟..中国荷斯坦奶牛CSN3基因多态性检测及其与产奶性状的关联分析[J].畜牧与兽医,2024,56(8):15-20,6.基金项目
安徽省科技重大专项(201903a06020013) （201903a06020013）
