畜牧与兽医2024，Vol.56Issue(8)：70-76,7.
鸭短喙侏儒综合征亚单位疫苗安全性及免疫效果评价
Evaluation on safety and immune effect of the subunit vaccine against duck short beak dwarfism syndrome
摘要
Abstract
This study was to evaluate the safety and immune effect of the subunit vaccine(rBac-JS01 VP2 strain)against duck short beak dwarfism syndrome(SBDS).Forty laying female ducks and 4 adult male ducks were obtained and randomly divided into 2 groups,with 20 laying female ducks and 2 adult male ducks in each group.The female ducks in the experimental group were injected with 0.5 mL of immune vaccine subcutaneously through the dorsal neck,and the female ducks in the negative control group were injected with 0.5 mL of PBS subcu-taneously through the dorsal neck.At the 2nd,4th,and 6th months after immunization,the eggs of each species were collected and incubated artificially.20 healthy male and 20 female ducks were randomly selected at each time period for SDBS-JS01 strain challenge.The weight and disease status of the ducks after the challenge were recorded,and the anal swabs of ducks were collected at 3,5,7,14,and 21 days after the challenge for detoxification.Two ducklings in each group were randomly dissected;then,pathological changes were observed,and the vi-rus distribution in each group was detected by PCR.In addition,at the 2nd,4th,and 6th months after the immunization,the blood and serum samples of all the laying ducks were collected,and the eggs of the laying ducks were collected to extract and purify yolk antibodies,and the titer of neutralizing antibodies against the virus was determined by the SBDS-JS01 strain.The results showed as follows:At the 2nd,4th,and 6th months after the immunization,the protection rates of the hatched ducks against SBDS-JS01 were 100%,95%and 90%,respectively.There was no significant difference in the body weight between the immune challenged group and the control group during the observation(P＞0.05),while the body weight of the ducks in the non-immune challenged group was significantly lower than that of the other groups(P＜0.01).After the challenge,the anal swabs of the ducks in the immune challenged group were negative at 2 months after the immunization,and the distribution of the virus in the tissues and organs were lower than those in the non-immune challenged group.At the 2nd,4th,and 6th months after the immunization,the titers of the serum neutralizing antibody against SBDS-JS01 were(10.51±1.59)log2,(9.46±1.27)log2,and(8.83±1.15)log2,and the titers of the yolk neutralizing antibody were(10.12±1.46)log2,(9.37±1.06)log2,and(8.49±1.21)log2,respectively.The titers of the serum neutralizing antibody and yolk neutralizing antibody were basically the same in different peri-ods;the rising and falling trends were the same,and it had a good correlation with the change of the protection level of the passive immune challenge.It was suggested that the prepared SBDS subunit vaccine had high safety and good immune effect,and might provide sustained and effective protection against duck short beak dwarfism syndrome.关键词
鸭短喙侏儒综合征/亚单位疫苗/攻毒保护/安全性/免疫效果Key words
SBDS/subunit vaccine/challenge protection/safety/immune effect分类
农业科技引用本文复制引用
甘辉群,吴双,傅宏庆,陈光明,孙飞,刘明生..鸭短喙侏儒综合征亚单位疫苗安全性及免疫效果评价[J].畜牧与兽医,2024,56(8):70-76,7.基金项目
江苏农牧科技职业学院畜禽产业核心技术创新项目(NSF2021ZR02) （NSF2021ZR02）
江苏省高等学校自然科学研究重大项目(21KJA230001) （21KJA230001）
