密旋链霉菌Act12对党参根系形态及药材品质的影响OA北大核心CSTPCDMEDLINE

As a kind of tonic Chinese medicine with dual use in medicine and food,there is a large market demanding for Codonopsis pilosula.Taking one-year-old C.pilosula seedlings as materials,we conducted a field experiment to examine the effect of compound fertilizer(750 kg·hm-2),organic fertilizer(15 t·hm-2)and Streptomyces pactum Act12 agent(9 t·hm-2Act12+10 t·hm-2 organic fertilizer)treatments on root morphology,secondary metabolite content and expression level of lobetyolin metabolic pathway gene of C.pilosula,to clarify the effects of three fertilizers on the root morphology and medicinal quality.Compared to the control(10 t·hm-2organ-ic fertilizer,conventional fertilization),three fertilization treatments could promote root growth and formation.All fertilization treatments promoted the accumulation of C.pilosula polysaccharides and secondary metabolites.Act12 agent significantly increased the content of lobetyolin,atractylenolideⅢ,and 5-hydroxymethylfurfural.The qRT-PCR analysis indicated that three fertilization treatments increased the expression level of lobetyolin metabolic path-way genes,with Act 12 agent treatment showing the most significant effect.Pearson correlation analysis demonstra-ted that the expression level of CpHCT and CpFAD genes was significantly positively correlated with atractylenolideⅢ content.In conclusion,three fertilization treatments could effectively improve the yield and quality of C.pilosu-la.Among the three treatments,Act 12 agent performed better than that of compound fertilizer and organic fertilizer,which was an effective measure to increase the yield and quality of C.pilosula.