抗ALS抑制剂类除草剂分子标记的开发及应用OA北大核心CSTPCD

Weeds seriously restrict rice production,and cultivating new varieties of rice with broad-spectrum herbicide resistance and acetyllactate synthase(ALS)inhibitors is one of an effective ways to deal with the harm of weeds in rice fields.The difference sites between the anti-ALS inhibitor herbicide Jinjing 818 and the sensitive herbicide Nipponbare ALS gene were identified,and specific molecular markers were developed.Herbicide spraying and molecular marker-assisted selection(MAS)were carried out on the hybrid offspring of Jinjing 818 and Jindao 372 with ALS-resistant genotypes as parents.Two pairs of molecular markers,818ALSF/R and HDALSF/R were developed,which could quickly distinguish three genotypes of homozygous dominant,heterozygous and homozygous genotypes,and were consistent with the herbicide spraying results.Two excellent rice germplasm resource materials,22S9 and 22S4,with excellent herbicide resistance,were bred.Germplasm resource materials with ALS herbicide resistance gene can be quickly screened out by MAS of hybrid offspring,and the screening efficiency in rice germplasm of anti-ALS inhibitor herbicides can be improved.