中国稻米2024，Vol.30Issue(4)：17-23,7.DOI:10.3969/j.issn.1006-8082.2024.04.004
抗ALS抑制剂类除草剂分子标记的开发及应用
Development of a Molecular Marker for Anti-ALS Inhibitor-like Herbicides and Its Applications
摘要
Abstract
Weeds seriously restrict rice production,and cultivating new varieties of rice with broad-spectrum herbicide resistance and acetyllactate synthase(ALS)inhibitors is one of an effective ways to deal with the harm of weeds in rice fields.The difference sites between the anti-ALS inhibitor herbicide Jinjing 818 and the sensitive herbicide Nipponbare ALS gene were identified,and specific molecular markers were developed.Herbicide spraying and molecular marker-assisted selection(MAS)were carried out on the hybrid offspring of Jinjing 818 and Jindao 372 with ALS-resistant genotypes as parents.Two pairs of molecular markers,818ALSF/R and HDALSF/R were developed,which could quickly distinguish three genotypes of homozygous dominant,heterozygous and homozygous genotypes,and were consistent with the herbicide spraying results.Two excellent rice germplasm resource materials,22S9 and 22S4,with excellent herbicide resistance,were bred.Germplasm resource materials with ALS herbicide resistance gene can be quickly screened out by MAS of hybrid offspring,and the screening efficiency in rice germplasm of anti-ALS inhibitor herbicides can be improved.关键词
ALS除草剂/乙酰乳酸合成酶/水稻/除草剂抗性Key words
ALS herbicide/acetolactate synthase/rice/herbicide resistance分类
农业科技引用本文复制引用
桑世飞,孙晓涵,姚国琴,马腾云,章怡静,郑阳阳,丰柳春,姬生栋..抗ALS抑制剂类除草剂分子标记的开发及应用[J].中国稻米,2024,30(4):17-23,7.基金项目
国家自然科学基金青年基金项目(32201823) （32201823）
新乡市重大科技专项(22ZD001) （22ZD001）
河南省科技攻关计划项目(212102110064) （212102110064）
