中国果菜2024，Vol.44Issue(7)：17-21,28,6.DOI:10.19590/j.cnki.1008-1038.2024.07.004
高效液相色谱法测定金银花中绿原酸及木犀草苷含量
Determination of Chlorogenic Acid and Galuteolin in Honeysuckle by HPLC
摘要
Abstract
This paper established a reverse high performance liquid chromatography(RP-HPLC)technique for the simultaneous determination of chlorogenic acid and galuteolin in honeysuckle.The ultrasonic extraction method was used for pretreatment,and the extraction solvent,extraction temperature and flow conditions were optimized.The results showed that the chlorogenic acid and galuteolin showed a good linear relationship over 0.5-100.0 mg/mL and correlation coefficients above 0.999.Recovery rate was 96%-108%,the RSD of repeatability was 2.05%and 2.44%,respectively.The method was simple and accurate,and it could be suitable for the determination of chlorogenic acid and galuteolin in fresh honeysuckle.关键词
金银花/绿原酸/木犀草苷/反向高效液相色谱法Key words
Honeysuckle/chlorogenic acid/galuteolin/reverse high performance liquid chromatography分类
医药卫生引用本文复制引用
曹宁,郑晓冬,闫新焕,刘雪梅,潘少香,谭梦男,王丽,王震,于素素,李志成..高效液相色谱法测定金银花中绿原酸及木犀草苷含量[J].中国果菜,2024,44(7):17-21,28,6.基金项目
山东省重点研发计划(重大科技创新工程)-金银花关键技术创新及应用示范(2022TZXD0036) （重大科技创新工程）
