中国感染控制杂志2024，Vol.23Issue(7)：833-839,7.DOI:10.12138/j.issn.1671-9638.20245300
香芹酚抑制高毒力肺炎克雷伯菌生物被膜形成机制的研究
Mechanism of carvacrol on inhibiting biofilm formation of hypervirulent Klebsiella pneumoniae
摘要
Abstract
Objective To explore the potential mechanism of carvacrol on inhibiting the formation of biofilm of hy-pervirulent Klebsiella pneumoniae(hvKP).Methods The possible mechanisms of carvacrol were analyzed based on the detection of its effects on the formation and morphology of biofilms,changes in extracellular polysaccharide and capsule polysaccharide content,as well as changes in the expression levels of biofilm-related genes rmpA2,mag A,mrkA,mrkB,and treC of hvKP.Results The minimum inhibitory concentration of carvacrol on hvKP was 512 μg/mL,with an obvious inhibitory effect on the biofilm formation of hvKP,presenting a concentration-depen-dent effect.Under the scanning electron microscope,it was observed that the biofilm structure was loose and the in-tercellular connections were not dense under the intervention of carvacrol.The Congo Red adsorption test and m-hydroxybiphenyl colorimetric method showed that carvacrol could reduce the content of capsule polysaccharides of hvKP,but didn't affect the total extracellular polysaccharide content.Fluorescence quantitative polymerase chain reaction(PCR)showed that under the effect of carvacrol at sub-inhibitory concentration,the synthesis of capsule polysaccharide,expression levels of sugar transport system and pili adhesion-related genes all decreased by more than 50％.Conclusion Carvacrol has a significant inhibitory effect on the formation of biofilm in hvKP,and its mechanism may be related to the decrease of synthesis of capsule polysaccharide as well as expression of biofilm-re-lated genes,such as sugar transport system and pili adhesion.关键词
香芹酚/高毒力肺炎克雷伯菌/生物被膜/荧光定量Key words
carvacrol/hypervirulent Klebsiella pneumoniae/biofilm/fluorescence quantification分类
医药卫生引用本文复制引用
魏春萍,向天新,刘洋,程娜,韩飞,周莉,刘蓬,魏丹丹..香芹酚抑制高毒力肺炎克雷伯菌生物被膜形成机制的研究[J].中国感染控制杂志,2024,23(7):833-839,7.基金项目
江西省中医药管理局科技计划项目(2022B1002) （2022B1002）
江西省科技厅项目(20232BAB206169) （20232BAB206169）
江西省教育厅项目(GJJ2200903) （GJJ2200903）
