中国人兽共患病学报2024，Vol.40Issue(6)：520-528,9.DOI:10.3969/j.issn.1002-2694.2024.00.086
马尔堡病毒密码子偏爱性分析
Codon usage bias of Marburg viruses
摘要
Abstract
The codon usage bias of the Marburg virus(MARV)genome and related influencing factors were investigated to provide a reference for preparation of vaccines and specific antibodies.In total,93 MARV coding sequences were retrieved from the GenBank database and analyzed with EMBOSS,Codon W,and SPSS 26.0,while plots were generated with SigmaPlot14.0 and GraphPad Prism 9.5.The effective number of codons(ENC values)of the MARV genomes for each protein ranged from 49.84 to 60.92,and the codon adaptation index was close to 0.7.Relative synonymous codon usage showed that GCA,AGA,AAU,GA A,GGA,AUU,CCU,and AC A were common high-frequency codons of seven proteins,and the codons with fa-voritism mostly ended with A/U.Neutral,ENC-Plot,and PR2 parity analyses indicated that the MARV codon usage prefer-ence was dominated by natural selection,followed by mutational pressure and various other factors.Comparisons of MARV gene codon usage frequency with four expression systems(Escherichia coli,yeast,human,and baculovirus)found differences in the codon usage frequency between MARV and E.coli.MARV codon usage favoritism was shaped by a combination of fac-tors,with natural selection playing a dominant role.The E.coli expression system was most suitable for in vitro expression of MARV proteins.关键词
马尔堡病毒/密码子偏爱性/自然选择Key words
Marburg virus/codon preference/natural selection分类
医药卫生引用本文复制引用
李健晴,刘奇,王聪,孟余..马尔堡病毒密码子偏爱性分析[J].中国人兽共患病学报,2024,40(6):520-528,9.基金项目
国家自然科学基金项目(No.81703573,No.81660337)和云南省地方本科高校基础研究联合专项(No.202301BA070001-040)联合资助 Supported by the National Natural Science Foundation of China(No.81703573,No.81660337)and the Joint Project on Basic Re-search of Local Undergraduate Universities in Yunnan Province(No.202301BA070001-040). （No.81703573,No.81660337）
