中国食品学报2024，Vol.24Issue(6)：24-32,9.DOI:10.16429/j.1009-7848.2024.06.003
乙醇对干酪乳酪杆菌Zhang甲基化转移酶突变体的影响
Effect of Ethanol on Methylated Transferase Mutant of Lacticaseibacillus casei Zhang
摘要
Abstract
Lactic acid bacteria will face ethanol stress when used to produce alcoholic beverages,so it is particularly important to enhance the ethanol stress tolerance of lactic acid bacteria in industrial breeding.Lacticaseibacillus casei Zhang(L.casei Zhang),isolated from traditional koumiss in Inner Mongolia,is a probiotic strain with excellent fermenta-tion performance.In this study,the differential metabolites between L.casei Zhang and its methylated transferase mutant were systematically analyzed by Liquid Chromatograph Mass Spectrometer(LC-MS)under 10％ethanol stress for 12 h.The aim of this study was to investigate the effects of DNA methylation on metabolites of L.casei Zhang under ethanol stress.t A total of 199 differential metabolites were identified between the methylated transferase mutant and the wild-type.Further enrichment analysis showed that the differential metabolites were mainly enriched in amino acid metabolism,ABC transporters,two-component system and pyruvate metabolism pathways.These findings have important implications for in-dustrial breeding of lactic acid bacteria.关键词
DNA甲基化/干酪乳酪杆菌/乙醇胁迫/代谢组学/液质联用Key words
DNA methylation/Lacticaseibacillus casei/ethanol stress/metabolomics/LC-MS引用本文复制引用
闫珈铭,张文羿..乙醇对干酪乳酪杆菌Zhang甲基化转移酶突变体的影响[J].中国食品学报,2024,24(6):24-32,9.基金项目
国家自然科学基金项目(31922071) （31922071）
内蒙古自然科学基金项目(2021ZD08) （2021ZD08）
