中国食品学报2024，Vol.24Issue(6)：369-379,11.DOI:10.16429/j.1009-7848.2024.06.032
洛阳传统绿豆酸浆细菌群落组成分析和功能预测
Bacterial Community Composition Analysis and Function Prediction of Luoyang Tradational Mung Bean Sour Pulp
摘要
Abstract
In order to explore the bacterial community composition of mung bean sour pulp,the core element of Luoyang traditional and special pulp noodles,the bacteria 16S rDNA of mung bean sour pulp from six pulp workshops in Lu-oyang was sequenced using Illumina MiSeq high-throughput sequencing technology.The results showed that 8 bacterial phyla were identified from 6 samples of mung bean sour pulp,involving 75 genera and 106 species;and Firmicutes is the dominant phylum(78.57％-97.17％),Lactobacillus(20.95％-90.36％)and Lactococcus(3.26％-62.68％)are the dominant genus,Lactobacillus oligofermentans,unclassified_Lactococcus are the corresponding dominant species,while Lactobacillus plantarum,Lactobacillus curvatus and Lactobacillus manihotivorans are common sub dominant bacteria.These dominant bacteria are all lactic acid bacteria,which have extensive prebiotic effects.The functional prediction of the bacterial community in mung bean sour pulp showed that the carbohydrate metabolism and amino acid metabolism abundance of the bacterial community are high,especially the abundance of flavor amino acid metabolic pathway and L-lactate dehydrogenase are relatively high.It was inferred that the unique acid and flavor of mung bean sour pulp was re-lated to the bacterial amino acid metabolism and lactic acid synthesis.The results of this study provided a theoretical basis for further study on the fermentation mechanism of mung bean sour pulp and development of corresponding starter.关键词
绿豆酸浆/高通量测序/细菌群落组成/功能预测Key words
mung bean sour pulp/high-throughput sequencing/bacterial community composition/function prediction引用本文复制引用
范鑫诺,李海阳,郑毅恒,李毅昊,侯颖,徐建强..洛阳传统绿豆酸浆细菌群落组成分析和功能预测[J].中国食品学报,2024,24(6):369-379,11.基金项目
国家自然科学基金项目(31400098) （31400098）
农业农村部农业微生物资源收集与保藏重点实验室开放基金项目(KLMRCP2021-06) （KLMRCP2021-06）
河南省科技攻关计划项目(202102110071) （202102110071）
