中国食品学报2024，Vol.24Issue(6)：392-401,10.DOI:10.16429/j.1009-7848.2024.06.034
内蒙古阿拉善驼乳制品中乳酸菌分离鉴定及微生物多样性研究
Isolation and Identification of Lactic Acid Bacteria and Microbial Diversity in Camel Dairy Products from Alxa Region,Inner Mongolia
摘要
Abstract
Inner Mongolia traditional dairy products are high quality medium for lactic acid bacteria.In order to preserve lactic acid bacteria resources in camel dairy products in Alxa region of Inner Mongolia and study the microbial diversity,the pure culture method and metagomic sequencing technology were respectively used to evaluate and preserve.Metage-nomic results showed that the microbial diversity of fresh camel milk was significantly higher than that of sour camel milk,and 157 species were identified from 18 samples.Lactococcus lactis(13.22％)and Acinetobacter guillouiae(10.65％)were the dominant species.The dominant species were Lactobacillus helveticus(72.66％),Lactobacillus kefi-ranofaciens(8.32％)and Lactococcus lactis(4.28％).A total of 121 strains of Lactobacillus were further isolated from the samples by pure culture and identified as 14 species of 4 genera,in which the dominant specie of fresh camel milk is Lactococcus lactis(62.00％).The dominant species were the Lactobacillus helveticus(40.00％)and the Lactobacillus ke-firanofaciens(19.00％),which were consistent with the metapomic results.The combination of metagomic sequencing and pure culture method effectively realized the microbial diversity analysis and Lactobacillus resource mining of camel milk samples,providing a theoretical basis for the industrial production of camel milk and the utilization of lactobacillus re-sources.关键词
驼乳/乳酸菌/微生物多样性/宏基因组Key words
camel milk/lactic acid bacteria/microbial diversity/metagenomic引用本文复制引用
苏茜,孙洁,杜萍,赵洁,于洁,陈永福..内蒙古阿拉善驼乳制品中乳酸菌分离鉴定及微生物多样性研究[J].中国食品学报,2024,24(6):392-401,10.基金项目
内蒙古自治区科技计划项目(2020GG0101) （2020GG0101）
内蒙古自治区高等学校创新团队发展计划项目(NMGIRT2220) （NMGIRT2220）
