液相色谱-串联质谱测定乳及乳制品中β-羟基-β-甲基丁酸钙含量研究OACSTPCD
Liquid Chromatography-Tandem Mass Spectrometry Determination of Milk and Dairy Products β-Hydroxyl Group-β-Calcium Methylbutyrate
目的:建立液相色谱-串联质谱法用于测定乳粉中β-羟基-β-甲基丁酸钙的含量.方法:样品经提取和沉淀蛋白后用液相色谱-串联质谱进行定性和定量测定.结果:该方法的检测线性范围为10.0～200.0 µg/L,线性相关系数≥0.999,方法检出限为50.0 µg/kg,在加标浓度为50.0 µg/kg～5.0 g/100 g条件下的回收率为78.4%～97.9%,精密度为1.58%～10.68%.结论:该方法采用液相色谱-串联质谱建立了一种简便、快速、准确的检测乳及乳制品中β-羟基-β-甲基丁酸钙含量的方法,检测效率高,前处理提取效率好,方法能够满足检测需要.
[Objective]A liquid chromatography tandem mass spectrometer(LC-MS/MS)method was established to determinate the content of calcium β-hydroxy-β-methylbutyrate(CaHMB)in dairy products.[Method]Samples were separated by ultra-high performance liquid chromatography after extraction and purification,and then qualitatively and quantitatively determined by mass spectrometer with electrospray ion(ESI)source.[Result]The linear range of this method was 10.0—200.0 µg/L and the linear correlation coefficient was above 0.99.The detection limit of this method was 50.0 µg/kg.The rate of recovery and repeatability was 78.4%—97.9%and 1.58%—10.68%respectively when the spiked concentration was 50.0 µg/kg—5.0 g/100 g.[Conclusion]This method used liquid chromatography tandem mass spectrometer(LC-MS/MS)to efficiently and rapidly detect the content of calcium β-hydroxy-β-methylbutyrate(CaHMB)in dairy products,which could meet the detection demands with the advantages of high testing efficiency and high extraction efficiency during pre-treatment.
黄雨晴;赵勇;林毅侃
上海市质量监督检验技术研究院/国家市场监管重点实验室(乳及乳制品检测与监控技术),上海 200233上海海洋大学食品学院,上海 201306
乳及乳制品β-羟基β-甲基丁酸钙液相色谱-串联质谱
dairy productcalcium β-hydroxy-β-methylbutyrate(CaHMB)LC-MS/MS
《中国食物与营养》 2024 (006)
51-55 / 5
上海市市场监督管理局科技项目(项目编号:2022-47).
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