中国动物传染病学报2024，Vol.32Issue(3)：50-56,7.
1株鸡毒支原体的分离鉴定及致病性研究
Isolation and Identification of a Mycoplasma gallisepticum Strain
张伯顺 1卜德新 2吕虹雨 3黄冬 1王睿智 1于春梅 1王进江 4丛雁方1
作者信息
- 1. 青岛蔚蓝生物股份有限公司,青岛 266000
- 2. 青岛动保国家工程技术研究中心有限公司,青岛 266000
- 3. 青岛蔚蓝生物制品有限公司,青岛 266000
- 4. 荣成市滕家畜牧兽医站,荣成 264300
- 折叠
摘要
Abstract
In order to study the genetic evolution and pathogenicity of a of Mycoplasma gallisepticum(MG)isolated from chicken farm,a specific band was amplified from suspicious air sac samples collected from a poultry farm in Laixi city of Shandong province using PCR assay with 16S rRNA primers.The purified strain SDMg01 was obtained by bacterial isolation and 16S rRNA gene sequencing.The typical colonies of"poached egg"shape were observed under the low power microscope.Biochemical identification results showed that SDMg01 strain fermented glucose but did not hydrolyze arginine and did not use urea.Phylogenetic analysis based on 16S rRNA sequences was performed by MEGA software.The result demonstrated that SDMg01 strain belonged to MG and clustered in the same branch with other MG strains.The homologies of 16S rRNA nucleotide sequences were 99.8％between SDMg01 strain and MG standard strain(PG31).Then,8-week-old SPF chickens were inoculated with SDMg01 strain.All chickens were observed for clinical signs and pathological changes of air sacs at day 14 post inoculation.The results showed that SDMg01 strain had high pathogenicity and as it induced typical airsacculitis.This study laid a foundation for the development of diagnostic reagents for MG and the screening of vaccine candidate strains.关键词
鸡毒支原体/16SrRNA/系统进化树/致病性Key words
Mycoplasma gallisepticum/16S rRNA/phylogenetic tree/pathogenicity分类
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张伯顺,卜德新,吕虹雨,黄冬,王睿智,于春梅,王进江,丛雁方..1株鸡毒支原体的分离鉴定及致病性研究[J].中国动物传染病学报,2024,32(3):50-56,7.
