中国现代中药2024，Vol.26Issue(7)：1119-1126,8.DOI:10.13313/j.issn.1673-4890.20230826001
霍山石斛枫斗溯源及不同贮藏年份挥发性成分分析
Provenance of Processed Caulis(Fengdou)of Dendrobium huoshanense and Volatile Components after Storage for Different Years
戴亚峰 1胡莉 2尚亮亮 1黄月纯 3纵瑞叶 1刘舒萍 2王诗文1
作者信息
- 1. 九仙尊霍山石斛股份有限公司,安徽 六安 237000
- 2. 广州中医药大学 中药学院,广东 广州 510006
- 3. 广州中医药大学 第一附属医院,广东 广州 510405
- 折叠
摘要
Abstract
Objective:To clarify the relationship between Dendrobium huoshanense and processed caulis(Fengdou)and explore the effects of storage years on the volatile components of D.huoshanense-derived Fengdou.Methods:Literature review and field investigation were carried out to clarify the relationship.Gas chromatography-mass spectrometry(GC-MS)was employed to analyze the volatile components of D.huoshanense-derived Fengdou stored for 0,2,4,and 6 years,and the relative content of each component was calculated according to the area of the chromatographic peak.Furthermore,the similarity of GC-MS fingerprints was analyzed.Results:Fengdou originated from D.huoshanense and should have a form with the basic characteristics of robust base and thin tip.The D.huoshanense-derived Fengdou was often referred to as"Lvmao Fengdou"mentioned in ancient books.A total of 25 common volatile components were identified from 12 batches of samples stored for different years,including 8 fatty acids,6 alkanes,4 alcohols,3 phenols,2 esters,and 2 other compounds.The GC-MS fingerprints showed the similarity of 0.851-0.997 among 12 batches of samples and 0.949-0.996 between the samples and reference fingerprint,which indicated a high similarity score.The results of principal component analysis(PCA)showed that samples stored for 0 and 2 years were clustered into one category,and those for 4 and 6 years were in the other category.The orthogonal partial least squares-discriminant analysis(OPLS-DA)was performed to identify the differential components,which were mainly fatty acids such as linoleic acid and linolenic acid.Conclusion:The basic characteristics of robust base and thin tip made D.huoshanense-derived Fengdou easy to be distinguished from Fengdou of other sources.The volatile components of D.huoshanense-derived Fengdou were similar after storage for different years,with differences only in the relative content.The volatile components were basically stable within 6 years of storage.关键词
霍山石斛枫斗/绿毛枫斗/贮藏年份/挥发性成分/气相色谱-质谱法Key words
Dendrobium huoshanense-derived Fengdou/Lvmao Fengdou/storage years/volatile components/gas chromatography-mass spectrometry(GC-MS)分类
医药卫生引用本文复制引用
戴亚峰,胡莉,尚亮亮,黄月纯,纵瑞叶,刘舒萍,王诗文..霍山石斛枫斗溯源及不同贮藏年份挥发性成分分析[J].中国现代中药,2024,26(7):1119-1126,8.
