赤水金钗石斛种源调查及HPLC指纹图谱比较OACSTPCD

Objective:To investigate the status of wild Dendrobium nobile in Chishui and establish HPLC fingerprints to preliminarily explore the differences between wild sources and imitative wild cultivation samples.Methods:A combination of literature review and field visits was adopted to investigate the wild D.nobile species and habitat conditions in Chishui city,Guizhou Province.High-performance liquid chromatography(HPLC)fingerprinting was used to analyze the wild sources,imitative wild cultivation samples,and mature dried and fresh products from six different bases.Results:Wild D.nobile species were found growing on ancient trees in Shibao township,Chishui city,with growth periods of approximately 50,30,and 20 years.There were 80-100 clusters of D.nobile on four ancient trees,with each cluster consisting of about 10-15 plants,and the total weight was 40-50 kg.The HPLC fingerprints of wild sources and imitative wild cultivation samples of D.nobile showed seven common characteristic peaks,with three of these peaks identified.The similarity index was above 0.940 for all samples.Conclusion:The wild D.nobile resources in Chishui are scarce,only found on a limited number of ancient trees.The HPLC fingerprints of imitative wild cultivation D.nobile and samples from different bases were highly similar to those of the wild sources,indicating that imitative wild cultivation D.nobile can maintain consistency with the wild germplasm.