中医药信息2024，Vol.41Issue(7)：13-17,5.DOI:10.19656/j.cnki.1002-2406.20240703
蜂针疗法对银屑病样小鼠模型皮肤中TRPV1、TRPM8含量的影响
Effect of Bee Sting Therapy on TRPV1 and TRPM8 Levels in the Skin of Psoriasis-like Mouse Model
摘要
Abstract
Objective:To observe the efficacy of bee sting therapy on an imiquimod-induced psoriasis-like mouse model and to investigate its effects on TRPV1 and TRPM8 levels.Methods:Forty BALB/c mice were randomly divided into a blank control group(3 mice)and a psoriasis model group(37 mice).After successful modeling,27 mice were randomly divided into the model group,the positive control group treated with bimekizumab cream,and the bee sting therapy group,with 9 mice in each group.From day 22 of the experiment,the positive control group was treated with topical bimekizumab cream once daily(10 mg per application),and the bee sting therapy group received bee sting therapy once a week(one sting per session,needle retained for 2 seconds)for 3 weeks.Skin lesions in each group were observed,and HE staining was used to observe the pathological status of the lesions.Immunohistochemistry was used to assess TRPV1 and TRPM8 expression in the lesions,ELISA was used to measure IL-17 levels,and RT-PCR was used to detect TRPV1 and TRPM8 mRNA expression in the lesions.Results:At the end of the experiment,serum IL-17 levels in the positive control and bee sting therapy groups decreased significantly compared to pre-treatment levels(P<0.05).Post-treatment IL-17 levels were also significantly lower than those in the model group(P<0.05).After bee sting therapy,TRPV1 expression in the skin lesions of model mice decreased significantly compared to the blank control group(P<0.05);however,there was no significant difference between the positive control and bee sting therapy groups compared to the blank control group(P>0.05).TRPM8 levels in the skin lesions of the bee needle therapy group significantly decreased compared to the model group(P<0.05),while the decrease in the positive control group was not significant(P>0.05).Conclusion:Bee sting therapy effectively improves skin lesions in psoriasis patients and imiquimod-induced psoriasis-like mice.Bee sting therapy was found to restore TRPV1 levels and reduce TRPM8 levels in skin lesions,suggesting a close relationship between the occurrence of psoriasis and the presence of thermosensitive channels in the skin.关键词
银屑病/蜂针疗法/辣椒素受体/瞬时受体电位M8离子通道Key words
Psoriasis/Bee sting therapy/TRPV1/TRPM8引用本文复制引用
朱凤,刘彬彬,陶迪生,雎恒春,朱建龙,朱子安,孙幸达..蜂针疗法对银屑病样小鼠模型皮肤中TRPV1、TRPM8含量的影响[J].中医药信息,2024,41(7):13-17,5.基金项目
江苏省中医药管理局项目(YB201924) （YB201924）
