中性粒细胞细胞外陷阱网通过促进破骨细胞生成加重根尖周炎炎症及骨吸收OACSTPCD

Objective:To explore the effect of osteoclast genesis regulated by neutrophil extracellular traps(NETs)on inflammation and bone resorption in periapical periodontitis.Methods:The periapical periodontitis model of C57BL/6 mice was established by direct exposure of the medullary cavity,and then the mice were randomly divided into model group and in-tervention group.The mice in the intervention group were subcutaneously injected with DNase I(10 mg/kg).Healthy mice were used as blank group.The levels of NETs in the periapical areas of the model group and the blank group were detected.The gingival index,periapical lesions,the number of neutrophils and osteoclasts in the lesion area,the distance from the ce-mento-enamel junction(CEJ)to the alveolar bone crest(ABC),and the levels of tumor necrosis factor(TNF)-α,interleukin(IL)-6 and IL-1β in the periapical tissues were compared.The protein levels of osteoprotegerin(OPG),receptor activator of nuclear factor-κB ligand(RANKL)and receptor activator of nuclear factor-κB(RANK)in the tissues around the lesion apex were detected.Results:The gingival index,periapical lesion area,the neutrophil counts/the content of NETs in the periapical lesion area and the levels of TNF-α,IL-6 and IL-1β mRNA in the periapical tissues of the model group were significantly higher than those of the blank group,while the above indexes of the intervention group were significantly lower than those of the model group(P<0.05).Compared with the blank group,the number of osteoclasts,the levels of RANKL and RANK pro-tein in surrounding tissues in the periapical lesion area of the model group were significantly increased,and the level of OPG protein was significantly decreased,while the number of osteoclasts and the levels of RANKL and RANK protein in the inter-vention group were significantly lower than those in the model group,and the level of OPG protein was significantly higher than that in the model group(P<0.05).Conclusion:The level of NETs in periapical tissues is significantly increased,which may promote periapical lesions and bone resorption by regulating osteoclast differentiation.