中国输血杂志2024，Vol.37Issue(8)：853-858,6.DOI:10.13303/j.cjbt.issn.1004-549x.2024.08.002
CD36蛋白B细胞抗原表位的生信预测及其多抗原肽的制备
Prediction of B cell epitopes of CD36 and preparation of MAP
摘要
Abstract
Objective To analyze the structure of CD36,search the possible B cell epitopes and prepare multi-antigen peptides(MAP)with B cell epitopes,so as to provide a preliminary experimental basis for the preparation of CD36 antibod-ies using MAP with B cell epitopes.Methods The potential B cell epitopes of CD36 were analyzed by bioinformatics meth-ods,including physical and chemical properties,secondary structure,potential phosphorylation and glycosylation sites.Eight-branch MAP with CD36 B cell epitopes were synthesized by FMOC using polylysine as the core matrix.The purity of MAPs was analyzed by reverse high-performance liquid chromatography chromatography(RP-HPLC),and the molecular weight of MAPs was determined by mass spectrometry.Results CD36 is a stable and hydrophilic alkaline protein,with multiple phosphorylation and glycosylation sites and strong antigenicity,and its secondary structure is mainly characterized by irregular curls.Four potential B cell epitopes were obtained and 4 MAPs containing potential B cell epitopes were pre-pared.RP-HPLC analysis showed that the purity of the MAPs were above 85%,and the molecular weight of 3 MAPs was consistent with the expected theoretical molecular weight.Conclusion CD36 on platelet has strong antigenicity.MAPs con-taining CD36 B cell epitopes can provide the experimental basis for the preparation and related research of CD36 antibodies.关键词
血小板/CD36/B细胞表位/MAP肽Key words
platelet/CD36/B cell epitope/MAP分类
医药卫生引用本文复制引用
刘静,徐秀章,丁浩强,邓晶,王嘉励,陈扬凯,夏文杰,叶欣..CD36蛋白B细胞抗原表位的生信预测及其多抗原肽的制备[J].中国输血杂志,2024,37(8):853-858,6.基金项目
广州市科技计划项目(2023A03J0997、2024A03J0227,2023A03J0552,2023A03J549) （2023A03J0997、2024A03J0227,2023A03J0552,2023A03J549）
广东省基础与应用基础研究基金自然科学基金项目(2024A1515012652) （2024A1515012652）
