南方农业学报2024，Vol.55Issue(6)：1639-1652,14.DOI:10.3969/j.issn.2095-1191.2024.06.009
花椒根腐病拮抗菌株W-1基因组测序及抑菌机理研究
Genome sequencing and antibacterial mechanism of Bacillus ve-lezensis strain W-1 against Zanthoxylum bungeanum root rot
摘要
Abstract
[Objective]The study aimed to analyze the whole genome sequence of Bacillus velezensis W-1 and to ex-plore its biocontrol mechanism against Zanthoxylum bungeanum root rot disease,providing a bioinformatics basis for its effective development and application,as well as theoretical support for its development as a biopesticide.[Method]The whole genome of strain W-1 was sequenced using the PacBio third-generation platform sequencing technology,and the gene function annotation and comparative genomics analysis were performed based on the sequencing results.Mycelia growth rate method was used to verify the effects of ethyl acetate extract of strain W-1 fermentation liquid on the myce-lium morphology of Z.bungeanum root rot pathogen,electrical conductivity of the pathogen spore suspension and nucleic acid content.[Result]The total genome length of strain W-1 was 4166284 bp,with a GC content of 46.32％,encoding 4037 protein genes.A total of 2935,3087,and 2185 genes were annotated in the GO,eggNOG and KEGG databases re-spectively.Fourteen secondary metabolite synthesis gene clusters were predicted in strain W-1,including 9 known gene clusters such as surfactin,butirosin A/butirosin B,plantazolicin,macrolactin H,bacillaene,fengycin,difficidin,bacilli-bactin and bacilysin,along with 5 unknown gene clusters.There were 53 unique gene families and 414 unique genes in strain W-1,showing close genetic relationship with the model strain B.velezensis FZB42.The antagonistic strain W-1 ex-tract greatly inhibited the mycelial growth of the Z.bungeanum root rot pathogen,with a minimum inhibitory concentra-tion(MIC)of 4.50 mg/mL and a minimum bactericidal concentration(MFC)of 9.00 mg/mL.The extract of strain W-1 could cause the exosmosis of intracellular contents from the pathogen mycelia.Compared with the blank control group,the relative electrical conductivity of the pathogen spore suspension and nucleic acid content increased by 57.63％and 64.91％respectively after treatment with an extract concentration of 9.00 mg/mL for 10 h.[Conclusion]B.velezensis W-1 can produce a variety of antibacterial substances.The ethyl acetate extract of its fermentation liquid can damage the integ-rity of the cell membrane of Z.bungeanum root rot pathogen,indicating its potential for development as a biopesticide and its promising application in the green prevention and control of Z.bungeanum root rot.关键词
花椒根腐病/贝莱斯芽孢杆菌/基因组测序/基因注释/抑菌机理Key words
Zanthoxylum bungeanum root rot/Bacillus velezensis/genome sequencing/gene annotation/antibacte-rial mechanism分类
农业科技引用本文复制引用
田凤鸣,陈强,何九军,张晓娜,王国斌..花椒根腐病拮抗菌株W-1基因组测序及抑菌机理研究[J].南方农业学报,2024,55(6):1639-1652,14.基金项目
Regional Project of National Natural Science Foundation of China(12061065) （12061065）
Gansu Science and Technology Fund for Youths(21JR7RK913) （21JR7RK913）
Longnan Science and Technology Project in 2021(2021-13) （2021-13）
Longnan Science and Technology Project in 2023(2023-S.QKJ-32) 国家自然科学基金地区基金项目(12061065) （2023-S.QKJ-32）
甘肃省青年科技基金项目(21JR7RK913) （21JR7RK913）
2021年度陇南市科技计划项目(2021-13) （2021-13）
2023年度陇南市科技计划项目(2023-S.QKJ-32) （2023-S.QKJ-32）
