现代食品科技2024，Vol.40Issue(7)：17-23,7.DOI:10.13982/j.mfst.1673-9078.2024.7.0857
菊粉对湿疹小鼠氧化还原状态和肠道菌群的影响
Effects of Inulin on Redox Status and Intestinal Microflora in Mouse Models of Eczema
摘要
Abstract
To explore the impact of inulin on the redox status and intestinal flora in mouse models of eczema,we utilized thirty male Kunming mice.These mice were randomly assigned to the control,model,and high-and low-dose inulin treatment groups,with interventions of varying insulin doses administered accordingly.Except for the control group,each group was sensitized with 2,4-dinitrochlorobenzene(DNCB)to establish eczema models.Inulin was added into the feed at mass fractions of 5%and 20%for high-and low-dose groups,respectively,for four weeks.HE staining was used to observe pathological changes in the skin and colon.The activity of total superoxide dismutase(T-SOD)and levels of malondialdehyde(MDA)in spleen and thymus were determined using biochemical methods.Feces were collected for 16S rRNA high-throughput sequencing to analyze the composition of gut microbiota in mice.The pathological results indicated that the changes in the skin and colon in mice were alleviated after inulin intervention.Comparison of the treatment groups with the model group revealed that inulin at a high dose significantly increased T-SOD and reduced MDA content in spleen and thymus(P<0.05).In addition,the abundance of Bifidobacterium,Allobaculum,and Olsenella in the low-dose group increased considerably(P<0.05),and the abundance of Allobaculum in the high-dose group increased significantly(P<0.05).Inulin at both low and high doses significantly reduced the abundance of Enterococcus.In short,inulin can effectively improve eczema by regulating gut microbiota and improving antioxidant capacity.关键词
菊粉/湿疹/2,4-二硝基氯苯/抗氧化/肠道菌群Key words
inulin/eczema/2,4-dinitrochlorobenzene/antioxidant/gut microbiota引用本文复制引用
蓝丹钰,谢小萍,莫玲,李晶晶..菊粉对湿疹小鼠氧化还原状态和肠道菌群的影响[J].现代食品科技,2024,40(7):17-23,7.基金项目
国家大学生创新创业训练计划项目(202010601033) （202010601033）
广西自然科学基金资助项目(2021GXNSFBA075038) （2021GXNSFBA075038）
广西高等学校千名中青年骨干教师培育计划(桂教教师[2020]58号) （桂教教师[2020]58号）
