现代食品科技2024，Vol.40Issue(7)：254-266,13.DOI:10.13982/j.mfst.1673-9078.2024.7.0695
龙眼及其制品中44种真菌毒素高通量检测方法的建立及其膳食暴露风险评估
Development of a High-throughput Detection Assay and Dietary Exposure Risk Assessment for 44 Mycotoxins in Longan and Its Products
摘要
Abstract
A high-throughput method based on UPLC-MS/MS was developed for the determination of 44 mycotoxins in longan and its products.The samples were extracted with 80%acetonitrile-water(V/V)solution containing 1%acetic acid,and purified by PSA and C18.A Waters ACQUITY UPLC BEH C18 column was used for separation,with 0.2%FA-acetonitrile and water-acetonitrile as the mobile phase for gradient elution.Quantification was performed using the internal standard method under MRM mode with positive and negative electropry ionization.Results showed that a good linear relationship was found for the 44 mycotoxins,with the correlation coefficients≥0.990 41,the limit of detection ranging within 0.003～0.7 μg/kg,and the limit of quantification being 0.01～2.0 μg/kg.The relative standard deviation(RSD)was 0.7%～9.7%(n=6),and the recoveries of the 44 mycotoxins at three spiking levels(low,intermediate and high levels)ranging within 70.7%～123.6%.The analyses of the 17 batches of longan and its products purchased from market revealed that the positive rate was 100%,with the concentrations of mycotoxins like 15-ADON,Fus X,PAT,TeA were relatively higher,indicating that they were the main contaminated mycotoxins during the growth,storage and processing processes of longan.Hazard Quotient(HQ)was used to assess the dietary exposure risk The assessment results showed that the HQ values of 21 mycotoxins were 0～0.012 37,which were all lower than 1,indicating no risk and little threat to human health.关键词
超高效液相色谱-串联质谱/真菌毒素/检测/膳食暴露风险评估Key words
UPLC-MS/MS/mycotoxins/detection/dietary exposure risk assessment引用本文复制引用
尹青春,陈小妹,周凌聿,陈春泉,高云慨,李备,梁成美,邓英林,邓浩..龙眼及其制品中44种真菌毒素高通量检测方法的建立及其膳食暴露风险评估[J].现代食品科技,2024,40(7):254-266,13.基金项目
国家市场监管重点实验室(热带果蔬质量与安全)自主研究课题(ZZ-2022003 （热带果蔬质量与安全）
ZZ-2023008) （）
国家市场监督管理总局科技计划项目(2022MK104) （2022MK104）
海南省重点研发计划项目(ZDYF2023XDNY082) （ZDYF2023XDNY082）
