现代食品科技2024，Vol.40Issue(7)：276-285,10.DOI:10.13982/j.mfst.1673-9078.2024.7.0830
超高效液相色谱-串联质谱法测定猪肉和淋巴肉中前列腺素E2含量
Determination of Prostaglandin E2 in Pork and Lymph Nodes by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry
摘要
Abstract
A method was developed for the determination of prostaglandin E2 in pork and lymph nodes based on ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Samples were extracted with acetonitrile,defatted with acetonitrile-saturated n-hexane,cleaned up by HLB solid-phase extraction columns,and the eluates were blown to near-dryness with nitrogen at 45℃and determined by re-solubilizing in a 50%acetonitrile solution containing 0.2%formic acid.The separation was conducted on a C18 column with 0.1%formic acid solution and acetonitrile as mobile phases,and the multiple reaction monitoring was performed in negative ion mode.The limit of detection for prostaglandin E2 in pork and its products was 10 μg/kg,and the limit of quantification was 20 μg/kg.The recoveries of prostaglandin E2 in the method were in the range of 90～120%,and the precision was below 15.0%.The accuracy and precision of the method met the requirements for quantitative analysis.The values of prostaglandin E2 in non-lymph node samples were below the limit of quantification using the established method,and the range of prostaglandin E2 in lymph node samples was 20.7～101.0 μg/kg.By determining the amount of prostaglandin E2 in a sample,it can be used as an aid in identifying lymph nodes.This method is highly accurate and generalizable,and it provides strong technical support for combating the illegal use of lymph nodes.关键词
前列腺素E2/淋巴肉/猪肉及其制品/质谱Key words
prostaglandin E2/lymph nodes/pork and its products/mass spectrometry引用本文复制引用
汪薇,吴婉琴,江丰,朱晓玲,张莉..超高效液相色谱-串联质谱法测定猪肉和淋巴肉中前列腺素E2含量[J].现代食品科技,2024,40(7):276-285,10.基金项目
国家市场监督管理总局科技计划项目(2021MK070) （2021MK070）
国家重点研发计划(2018YFC1602303). （2018YFC1602303）
