食品与机械2024，Vol.40Issue(7)：163-168,230,7.DOI:10.13652/j.spjx.1003.5788.2024.60072
顺序式模拟移动床色谱高效纯化低聚异麦芽糖应用研究
Application on efficient purification of isomaltooligosaccharides by sequential simulated moving bed chromatography
摘要
Abstract
[Objective]To improve the production efficiency of the oligosaccharide industry.[Methods]Isomaltooligosaccharides 50 syrup was used as the raw material,and purified by sequential simulated moving bed chromatography.Potassium ion resin was selected as the suitable separation medium by preparative chromatography single column experiment.Then,based onthe principle of material balance and the basic principle of sequential simulated moving bed,the initial operating parameters of separation process were approximately established.Further the separation conditions were optimized.[Results]The best technical parameters were as follows:feed refraction was 60％and column temperature was 65 ℃.At the same time quantity of flow in the entrance and eluent were 455 mL/h and 682 mL/h,respectively.The velocity of flow in the entrance and eluent were 37.4 mL/min and 29.6 mL/min,respectively.Besides,the circulating flow was kept within 364 mL/h.Finally,the outlet refraction of isomaltooligosaccharides was 20.40％with the purity of 90.20％and the yield of 85.50％.[Conclusion]The sequential simulated moving bed chromatographic separation equipment independently developed by the laboratory can efficiently purify isomaltooligosaccharides.关键词
顺序式模拟移动床/色谱分离/纯化/低聚异麦芽糖Key words
sequential simulated moving bed/chromatographic separation/purification/isomaltooligosaccharides引用本文复制引用
阮圣慧,郑喜群,刘晓兰,李良玉,姜彩霞..顺序式模拟移动床色谱高效纯化低聚异麦芽糖应用研究[J].食品与机械,2024,40(7):163-168,230,7.基金项目
黑龙江省"百千万"工程科技重大专项(编号:2019ZX06B02-1) （编号:2019ZX06B02-1）
黑龙江八一农垦大学研究生创新科研项目(编号:YJSCX2022-Y47) （编号:YJSCX2022-Y47）
