金丝皇菊组培快繁体系建立OA

Dendranthema morifolium with high medicinal value,is the important tea chrysanthemum variety.In order to establishment of a rapid seedling propagation system,this study took Dendranthema morifolium as the experimental material.Tissue culture was used.By studying the effects of two hormone NAA and 6-BA,on the healing tissues,shoot tips,cluster buds,and roots.The results showed that the most suitable medium for inducing leaves to form healing tissue was MS+30 g·L-1 sucrose+0.5 mg·L-1NAA+l.0 mg·L-16-BA,and the healing induction rate reached 100％after 30 days.The optimal shoot tip induction medium was MS+30 g·L-1 sucrose+0.1 mg·L-1NAA+1.5 mg·L-16-BA,which induced the formation of indeterminate buds with strong activity and high differen-tiation rate.The best proliferation medium was MS+30 g·L-1 sucrose+1.0 mg·L-16-BA+0.1 mg·L-1NAA.The rooting rate in MS medium without hormone reached 100％after 15 days,and showed great growth with greener leaves and stronger stems.In conclusion,this study lays the foundation for the study of non-toxic seedling breeding of Dendranthema morifolium,which is of great significance for the sustainable development of chrysanthemum industry for tea in the countryside.