天津农业科学2024，Vol.30Issue(7)：1-6,12,7.DOI:10.3969/j.issn.1006-6500.2024.07.001
金丝皇菊组培快繁体系建立
A Preliminary Study on the Tissue Culture Technology of Dendranthema morifolium
摘要
Abstract
Dendranthema morifolium with high medicinal value,is the important tea chrysanthemum variety.In order to establishment of a rapid seedling propagation system,this study took Dendranthema morifolium as the experimental material.Tissue culture was used.By studying the effects of two hormone NAA and 6-BA,on the healing tissues,shoot tips,cluster buds,and roots.The results showed that the most suitable medium for inducing leaves to form healing tissue was MS+30 g·L-1 sucrose+0.5 mg·L-1NAA+l.0 mg·L-16-BA,and the healing induction rate reached 100％after 30 days.The optimal shoot tip induction medium was MS+30 g·L-1 sucrose+0.1 mg·L-1NAA+1.5 mg·L-16-BA,which induced the formation of indeterminate buds with strong activity and high differen-tiation rate.The best proliferation medium was MS+30 g·L-1 sucrose+1.0 mg·L-16-BA+0.1 mg·L-1NAA.The rooting rate in MS medium without hormone reached 100％after 15 days,and showed great growth with greener leaves and stronger stems.In conclusion,this study lays the foundation for the study of non-toxic seedling breeding of Dendranthema morifolium,which is of great significance for the sustainable development of chrysanthemum industry for tea in the countryside.关键词
金丝皇菊/组织培养/植物激素/扩繁Key words
Dendranthema morifolium Jinsihuangju/tissue culture/phytohormone/propagation分类
农业科技引用本文复制引用
高敏,霍雯雯,陈汀,李鹏鹏,何丽波,许璐..金丝皇菊组培快繁体系建立[J].天津农业科学,2024,30(7):1-6,12,7.基金项目
湖南省教育厅科学研究重点项目(22A0143) （22A0143）
长沙市软科学研究计划项目(kh2302045) （kh2302045）
