蒲葵子粗多糖脱蛋白方法及其抑制破骨细胞分化活性OA

This study primarily aimed to explore the optimal protein removal method for polysaccharide of the seed of Livistona chinensis.Using protein clearance rate and polysaccharide retention rate as evaluation indicators,the effects of Sevage method,enzymatic method,and the combination of enzyme and Sevage method on the protein removal from polysaccharide of the seeds were investigated.Additionally,the influence of the obtained polysaccharide of the seeds on RANKL-induced osteoclast differentiation in RAW264.7 cells was explored.The protein removal rates of polysaccharide of the seed using the Sevage method,enzymatic method,and the combined method were 78.85%,82.40%,and 82.73%,respectively.The polysaccharide retention rates were 72.42%,92.16%,and 96.72%,respectively.Treatment of RANKL-induced RAW264.7 osteoclast models with polysaccharide of the seed of L.chinensis obtained using the optimal protein removal method revealed that it could reduce the number of osteoclasts generated and decrease their TRAP activity.Therefore,the combination of enzyme and Sevage for protein removal was a relatively effective method that could significantly reduce the protein content in polysaccharide of L.chinensis seed while maintaining a high polysaccharide retention rate.The obtained polysaccharide could also inhibit RANKL-induced osteoclast differentiation in RAW264.7 cells,providing theoretical support for the development of new anti-osteoporosis drugs.