新疆图木舒克市牛梨形虫感染情况调查OA

In order to investigate the infection status of bovine Piroplasma in Tumushuke,Xinjiang,862 bovine whole blood samples taken from 10 sampling sites were detected for Piroplasma by PCR,and sequence comparison and phylogenetic tree construction for the positive products were conducted.The results showed that the total infection rate of bovine Piroplasma was 41.53%(358/862),a new case infected with Theileria orientalis was found,and all the others were infected with T.annulata,but none was with Babesia;it was found that,based on the detection results of different sampling sites,the positive rate of Piroplasma was the highest in site 4(64.91%),then in site 9(61.11%),lower in the sites of 1 and 3,but none Piroplasma was found in site 10;based on the detection results of different times,it was showed that the positive rate of Piroplasma tended to decrease in sites of 1,2,3,6,7 and 9,but increase in sites 4 and 5;phylogenetic tree showed that T.annulata was 100%identical to the strain of Xinjiang origin(EU083799),both of which were clustered as a branch on the evolutionary tree,and T.orientalis was 99%identical to the strain(ON148462)isolated in Bosnia and Herzegovina,indicating a close genetic relationship.In conclusion,Piroplasma was widely distributed in cattle in Tumushuke,but its positive rate depended on different sites;T.annulata is the dominant Piroplasma species in this area.Farms were recommended to prepare targeted plans to prevent and control Piroplasma according to local conditions and status of infection,and to improve their monitoring over bovine Piroplasma.