浙江医学2024，Vol.46Issue(15)：1573-1579,后插1,8.DOI:10.12056/j.issn.1006-2785.2024.46.15.2024-1252
基于黏附素HpaA B细胞表位的多抗原肽疫苗的免疫原性鉴定
Preparing a multi-antigen peptide vaccine based on B-cell epitope of Helicobacter pylori adhesin HpaA and determining its immunogenicity
摘要
Abstract
Objective To prepare a multiple antigenic polypetide(MAP)vaccine based on the B-cell epitope of Helicobacter pylori(Hp)adhesin HpaA and to determine its immunogenicity.Methods Amino acid and nucleotide sequences of HpaA were obtained from GenBank.Epitope analysis softwares were utilized for HpaA B-cell epitope prediction.An 8-branch MAP was synthesized and used to immunize white-haired black eye(WHBE)rabbits.The affinity of MAP and rabbit anti-Hp polyclonal antibodies in vitro was determined,and the serum antibody titer and antibody specificity in vivo were detected.Results The dominant B cell epitopes of HpaA were located in the 130-142 and 212-219 amino acid segments,the 8-branched MAP1 and MAP2 were synthesized according to above B cell epitopes.Both MAP1 and MAP2 showed binding ability to rabbit anti-Hp polyclonal antibodies in vitro,while MAP1 exhibited higher affinity.Only MAP1 induced immune response and produced high-titer specific antibody in vivo.Conclusion The dominant B-cell epitope is located in 130-142 amino acid segment of HpaA,the corresponding MAP was synthesized and the vaccine was prepared in the study,which demonstrates strong immunogenicity.关键词
幽门螺杆菌/黏附素HpaA/B细胞表位/多抗原肽/免疫原性Key words
Helicobacter pylori/Adhesin HpaA/B-cell epitope/Multi antigenic polypeptide/Immunogenicity引用本文复制引用
孙艳,李瑞芳,格桑卓玛,冯明旨,张骏..基于黏附素HpaA B细胞表位的多抗原肽疫苗的免疫原性鉴定[J].浙江医学,2024,46(15):1573-1579,后插1,8.基金项目
浙江省医药卫生重大科技计划项目(WKJ-ZJ-2018) （WKJ-ZJ-2018）
浙江省中医药科技计划项目(2023ZL266) （2023ZL266）
