植物学报2024，Vol.59Issue(4)：558-573,16.DOI:10.11983/CBB23129
SIHVA22I基因调节番茄耐旱性
Investigation of the Regulation of Drought Tolerance by the SIHVA22I Gene in Tomato
摘要
Abstract
Plants inevitably face a multitude of abiotic stresses during their growth and development stages.Drought stress significantly hampers crop growth and reduces yield.The plant HVA22 protein is characterized by the TB2/DP1 structural domain and is implicated in the modulation of plant growth,development,and responses to abiotic stress.However,its precise function in the context of drought stress response in tomato remains to be elucidated.Therefore,in this study,we investigated the functional role of the tomato SIHVA22I gene in drought tolerance.The results showed that the amino acid sequence of SIHVA22I exhibits a higher degree of sequence similarity to that of homologous HVA22I proteins found in other dicotyledonous plants.Furthermore,the expression pattern analysis revealed a significant upregulation of the SIHVA22I gene in response to drought stress and phytohormones(ABA and MeJA).Moreover,the function of the SIHVA22I gene in drought tolerance was subsequently verified by yeast heterologous expression and silencing of the endogenous SIHVA22I gene in tomato via virus-induced gene silencing.The silenced plants exhibited higher H2O2 and malondialdehyde contents,as well as lower O2-·scavenging after drought treatment.Moreover,the activities of superoxide dismutase,peroxidase,catalase,and ascorbate peroxidase were significantly decreased in the silenced plants compared to those in the control plants.Collectively,these results indicate that the SIHVA22I gene plays an important role in tomato resistance to drought stress.关键词
番茄/SIHVA22I/干旱胁迫/病毒诱导基因沉默(VIGS)/基因表达Key words
tomato/SIHVA22I/drought stress/virus-induced gene silencing(VIGS)/gene expression引用本文复制引用
赵来鹏,王柏柯,杨涛,李宁,杨海涛,王娟,闫会转..SIHVA22I基因调节番茄耐旱性[J].植物学报,2024,59(4):558-573,16.基金项目
农业科技创新稳定支持项目(No.xjnkywdzc-2023001)、自治区重点研发计划(No.2022B02032-2)、新疆特色果蔬基因组研究与遗传改良重点实验室开放课题(2023)和新疆维吾尔自治区蔬菜产业技术体系(No.XJARS-07) （No.xjnkywdzc-2023001）
