植物学报2024，Vol.59Issue(4)：613-625,13.DOI:10.11983/CBB23178
毛建草愈伤组织诱导及植株再生
Callus Induction and Plant Regeneration of Dracocephalum rupestre
摘要
Abstract
Dracocephalum rupestre is an important medicinal plant.However,its leaf explant regeneration system has yet to be established.Here we investigated the impact of plant growth regulators on callus induction,differentiation,ad-ventitious bud proliferation,and rooting using field planted leaves and tissue-cultured seedling leaves of D.rupestre as explants.We found that the optimal medium for inducing callus from field planted leaves was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 2,4-D+1.0 mg·L-1 IAA,achieving an induction rate of 84.51％.For adventitious bud differentiation,the pre-ferred medium comprised MS+3.0 mg·L-1 6-BA+0.5 mg·L-1 TDZ+0.5 mg·L-1 IAA,resulting in a differentiation rate of 66.37％.Similarly,for inducing callus from tissue-cultured seedling leaves,the optimal medium included MS+2.0 mg·L-16-BA+0.1 mg·L-1 2,4-D+0.5 mg·L-1 IAA,with an induction rate of 86.73％.The medium conductive to adventitious bud differentiation consisted of MS+2.0 mg·L-1 6-BA+2.0 mg·L-1 TDZ+0.05 mg·L-1 IAA,yielding a differentiation rate of 53.48％.Furthermore,the appropriate medium for adventitious bud proliferation was MS+2.0 mg·L-1 6-BA+0.05 mg·L-1 NAA,achieving a proliferation rate of 83.57％.The rooting medium was 1/2MS+0.1 mg·L-1 NAA+0.1 mg·L-1 IBA,resulting in a rooting rate of 86.97％.Tissue-cultured seedlings exhibited optimal growth in a mixed substrate of peat and vermicu-lite at a ratio of 1∶1(v/v).This study successfully established a leaf explant regeneration system for D.rupestre,providing valuable technical support for the conservation and rapid propagation of its germplasm resources.关键词
毛建草/愈伤组织诱导/分化/增殖/生根Key words
Dracocephalum rupestre/callus induction/differentiation/proliferation/rooting引用本文复制引用
田旭平,岳康杰,王佳丽,刘慧欣,史子尹,亢红伟..毛建草愈伤组织诱导及植株再生[J].植物学报,2024,59(4):613-625,13.基金项目
山西农业大学科技创新基金(No.2020BQ37) （No.2020BQ37）
